High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function

M. Adam, Henryk Urbanski, V. T. Garyfallou, U. Welsch, F. M. Köhn, J. Ullrich Schwarzer, L. Strauss, M. Poutanen, A. Mayerhofer

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Decorin (DCN), a component of the extracellular matrix of the peritubular wall and the interstitial areas of the human testis, can interact with growth factor (GF) signalling, thereby blocking downstream actions of GFs. In the present study the expression and regulation of DCN using both human testes and two experimental animal models, namely the rhesus monkey and mouse, were examined. DCN protein was present in peritubular and interstitial areas of adult human and monkey testes, while it was almost undetectable in adult wild type mice. Interestingly, the levels and sites of testicular DCN expression in the monkeys were inversely correlated with testicular maturation markers. A strong DCN expression associated with the abundant connective tissue of the interstitial areas in the postnatal through pre-pubertal phases was observed. In adult and old monkeys the DCN pattern was similar to the one in normal human testes, presenting strong expression at the peritubular region. In the testes of both infertile men and in a mouse model of inflammation associated infertility (aromatase-overexpressing transgenic mice), the fibrotic changes and increased numbers of tumour necrosis factor (TNF)-α-producing immune cells were shown to be associated with increased production of DCN. Furthermore, studies with human testicular peritubular cells isolated from fibrotic testis indicated that TNF-α significantly increased DCN production. The data, thus, show that an increased DCN level is associated with impaired testicular function, supporting our hypothesis that DCN interferes with paracrine signalling of the testis in health and disease.

    Original languageEnglish (US)
    Pages (from-to)550-561
    Number of pages12
    JournalInternational Journal of Andrology
    Volume35
    Issue number4
    DOIs
    StatePublished - Aug 2012

    Fingerprint

    Decorin
    Proteoglycans
    Extracellular Matrix
    Testis
    Haplorhini
    Tumor Necrosis Factor-alpha
    Paracrine Communication
    Aromatase
    Macaca mulatta
    Connective Tissue
    Infertility
    Transgenic Mice
    Intercellular Signaling Peptides and Proteins
    Animal Models

    Keywords

    • Development
    • Fibrosis
    • Infertility
    • Inflammation
    • Mouse < animal models
    • Primates < animal models
    • Proteoglycan
    • Testis

    ASJC Scopus subject areas

    • Urology
    • Reproductive Medicine
    • Endocrinology, Diabetes and Metabolism

    Cite this

    High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function. / Adam, M.; Urbanski, Henryk; Garyfallou, V. T.; Welsch, U.; Köhn, F. M.; Ullrich Schwarzer, J.; Strauss, L.; Poutanen, M.; Mayerhofer, A.

    In: International Journal of Andrology, Vol. 35, No. 4, 08.2012, p. 550-561.

    Research output: Contribution to journalArticle

    Adam, M, Urbanski, H, Garyfallou, VT, Welsch, U, Köhn, FM, Ullrich Schwarzer, J, Strauss, L, Poutanen, M & Mayerhofer, A 2012, 'High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function', International Journal of Andrology, vol. 35, no. 4, pp. 550-561. https://doi.org/10.1111/j.1365-2605.2011.01225.x
    Adam, M. ; Urbanski, Henryk ; Garyfallou, V. T. ; Welsch, U. ; Köhn, F. M. ; Ullrich Schwarzer, J. ; Strauss, L. ; Poutanen, M. ; Mayerhofer, A. / High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function. In: International Journal of Andrology. 2012 ; Vol. 35, No. 4. pp. 550-561.
    @article{e079dc3e19d54dfdbb6c51dd8b002d6a,
    title = "High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function",
    abstract = "Decorin (DCN), a component of the extracellular matrix of the peritubular wall and the interstitial areas of the human testis, can interact with growth factor (GF) signalling, thereby blocking downstream actions of GFs. In the present study the expression and regulation of DCN using both human testes and two experimental animal models, namely the rhesus monkey and mouse, were examined. DCN protein was present in peritubular and interstitial areas of adult human and monkey testes, while it was almost undetectable in adult wild type mice. Interestingly, the levels and sites of testicular DCN expression in the monkeys were inversely correlated with testicular maturation markers. A strong DCN expression associated with the abundant connective tissue of the interstitial areas in the postnatal through pre-pubertal phases was observed. In adult and old monkeys the DCN pattern was similar to the one in normal human testes, presenting strong expression at the peritubular region. In the testes of both infertile men and in a mouse model of inflammation associated infertility (aromatase-overexpressing transgenic mice), the fibrotic changes and increased numbers of tumour necrosis factor (TNF)-α-producing immune cells were shown to be associated with increased production of DCN. Furthermore, studies with human testicular peritubular cells isolated from fibrotic testis indicated that TNF-α significantly increased DCN production. The data, thus, show that an increased DCN level is associated with impaired testicular function, supporting our hypothesis that DCN interferes with paracrine signalling of the testis in health and disease.",
    keywords = "Development, Fibrosis, Infertility, Inflammation, Mouse < animal models, Primates < animal models, Proteoglycan, Testis",
    author = "M. Adam and Henryk Urbanski and Garyfallou, {V. T.} and U. Welsch and K{\"o}hn, {F. M.} and {Ullrich Schwarzer}, J. and L. Strauss and M. Poutanen and A. Mayerhofer",
    year = "2012",
    month = "8",
    doi = "10.1111/j.1365-2605.2011.01225.x",
    language = "English (US)",
    volume = "35",
    pages = "550--561",
    journal = "International Journal of Andrology",
    issn = "0105-6263",
    publisher = "Wiley-Blackwell",
    number = "4",

    }

    TY - JOUR

    T1 - High levels of the extracellular matrix proteoglycan decorin are associated with inhibition of testicular function

    AU - Adam, M.

    AU - Urbanski, Henryk

    AU - Garyfallou, V. T.

    AU - Welsch, U.

    AU - Köhn, F. M.

    AU - Ullrich Schwarzer, J.

    AU - Strauss, L.

    AU - Poutanen, M.

    AU - Mayerhofer, A.

    PY - 2012/8

    Y1 - 2012/8

    N2 - Decorin (DCN), a component of the extracellular matrix of the peritubular wall and the interstitial areas of the human testis, can interact with growth factor (GF) signalling, thereby blocking downstream actions of GFs. In the present study the expression and regulation of DCN using both human testes and two experimental animal models, namely the rhesus monkey and mouse, were examined. DCN protein was present in peritubular and interstitial areas of adult human and monkey testes, while it was almost undetectable in adult wild type mice. Interestingly, the levels and sites of testicular DCN expression in the monkeys were inversely correlated with testicular maturation markers. A strong DCN expression associated with the abundant connective tissue of the interstitial areas in the postnatal through pre-pubertal phases was observed. In adult and old monkeys the DCN pattern was similar to the one in normal human testes, presenting strong expression at the peritubular region. In the testes of both infertile men and in a mouse model of inflammation associated infertility (aromatase-overexpressing transgenic mice), the fibrotic changes and increased numbers of tumour necrosis factor (TNF)-α-producing immune cells were shown to be associated with increased production of DCN. Furthermore, studies with human testicular peritubular cells isolated from fibrotic testis indicated that TNF-α significantly increased DCN production. The data, thus, show that an increased DCN level is associated with impaired testicular function, supporting our hypothesis that DCN interferes with paracrine signalling of the testis in health and disease.

    AB - Decorin (DCN), a component of the extracellular matrix of the peritubular wall and the interstitial areas of the human testis, can interact with growth factor (GF) signalling, thereby blocking downstream actions of GFs. In the present study the expression and regulation of DCN using both human testes and two experimental animal models, namely the rhesus monkey and mouse, were examined. DCN protein was present in peritubular and interstitial areas of adult human and monkey testes, while it was almost undetectable in adult wild type mice. Interestingly, the levels and sites of testicular DCN expression in the monkeys were inversely correlated with testicular maturation markers. A strong DCN expression associated with the abundant connective tissue of the interstitial areas in the postnatal through pre-pubertal phases was observed. In adult and old monkeys the DCN pattern was similar to the one in normal human testes, presenting strong expression at the peritubular region. In the testes of both infertile men and in a mouse model of inflammation associated infertility (aromatase-overexpressing transgenic mice), the fibrotic changes and increased numbers of tumour necrosis factor (TNF)-α-producing immune cells were shown to be associated with increased production of DCN. Furthermore, studies with human testicular peritubular cells isolated from fibrotic testis indicated that TNF-α significantly increased DCN production. The data, thus, show that an increased DCN level is associated with impaired testicular function, supporting our hypothesis that DCN interferes with paracrine signalling of the testis in health and disease.

    KW - Development

    KW - Fibrosis

    KW - Infertility

    KW - Inflammation

    KW - Mouse < animal models

    KW - Primates < animal models

    KW - Proteoglycan

    KW - Testis

    UR - http://www.scopus.com/inward/record.url?scp=84863879971&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84863879971&partnerID=8YFLogxK

    U2 - 10.1111/j.1365-2605.2011.01225.x

    DO - 10.1111/j.1365-2605.2011.01225.x

    M3 - Article

    VL - 35

    SP - 550

    EP - 561

    JO - International Journal of Andrology

    JF - International Journal of Andrology

    SN - 0105-6263

    IS - 4

    ER -