High level of interleukin-10 production by the activated T cell population within the rheumatoid synovial membrane

Shara B A Cohen, Peter D. Katsikis, Cong-Qiu Chu, Henrike Thomssen, Louise M C Webb, Ravinder N. Maini, Marco Londei, Marc Feldmann

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Objective. To characterize the cytokine profile of the activated T cell population derived from the synovial membrane of rheumatoid arthritis (RA) patients. Methods. Interleukin-2 (IL-2) was used to select for in vivo- activated T cells from the synovial membrane of 2 patients with RA, and the cells were cloned nonspecifically. The cytokine production profile of these clones was compared with that of clones derived from peripheral blood monocytes (PBM) by stimulating all clones for 24 hours with immobilized anti- CD3 (coated at 10 μg/ml) or phorbol-12-myristate-13-acetate (10 ng/ml) plus soluble anti-CD3 (1 μg/ml). Interferon-γ (IFNγ), IL-4, and IL-10, the cytokines that discriminate between Th1 and Th2 cells and are involved in immunoregulation, were assayed by enzyme-linked immunosorbent assay. Results. There was a difference in the cytokines produced by the clones derived from the rheumatoid membranes compared with clones derived from the periphery. Clones derived from both membranes and PBM were mostly IFNγ-producers, i.e., either a Th0 or a Th1 profile. There was a high proportion of IFNγ/high IL- 10-producing cells derived from the joint, but not from the periphery. Of clones derived from the synovial membrane of each of 2 RA patients, 100% and 50% produced both 1-10 ng/ml IFNγ and >7 ng/ml IL-10, compared with

Original languageEnglish (US)
Pages (from-to)946-952
Number of pages7
JournalArthritis and Rheumatism
Volume38
Issue number7
DOIs
StatePublished - Jul 1995
Externally publishedYes

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Synovial Membrane
Interleukin-10
Clone Cells
T-Lymphocytes
Interferons
Population
Cytokines
Rheumatoid Arthritis
Monocytes
Th2 Cells
Th1 Cells
Membranes
Interleukin-4
Interleukin-2
Acetates
Joints
Enzyme-Linked Immunosorbent Assay
Cell Membrane

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Cohen, S. B. A., Katsikis, P. D., Chu, C-Q., Thomssen, H., Webb, L. M. C., Maini, R. N., ... Feldmann, M. (1995). High level of interleukin-10 production by the activated T cell population within the rheumatoid synovial membrane. Arthritis and Rheumatism, 38(7), 946-952. https://doi.org/10.1002/art.1780380710

High level of interleukin-10 production by the activated T cell population within the rheumatoid synovial membrane. / Cohen, Shara B A; Katsikis, Peter D.; Chu, Cong-Qiu; Thomssen, Henrike; Webb, Louise M C; Maini, Ravinder N.; Londei, Marco; Feldmann, Marc.

In: Arthritis and Rheumatism, Vol. 38, No. 7, 07.1995, p. 946-952.

Research output: Contribution to journalArticle

Cohen, SBA, Katsikis, PD, Chu, C-Q, Thomssen, H, Webb, LMC, Maini, RN, Londei, M & Feldmann, M 1995, 'High level of interleukin-10 production by the activated T cell population within the rheumatoid synovial membrane', Arthritis and Rheumatism, vol. 38, no. 7, pp. 946-952. https://doi.org/10.1002/art.1780380710
Cohen, Shara B A ; Katsikis, Peter D. ; Chu, Cong-Qiu ; Thomssen, Henrike ; Webb, Louise M C ; Maini, Ravinder N. ; Londei, Marco ; Feldmann, Marc. / High level of interleukin-10 production by the activated T cell population within the rheumatoid synovial membrane. In: Arthritis and Rheumatism. 1995 ; Vol. 38, No. 7. pp. 946-952.
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