High-level iodination of monoclonal antibody fragments for radiotherapy

J. M. Ferens, K. A. Krohn, P. L. Beaumier, J. P. Brown, I. Hellström, K. E. Hellström, J. A. Carrasquillo, S. M. Larson

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38 Scopus citations

Abstract

Two different murine monoclonal antibody Fab fragments specific for p97, a melanoma-associated antigen, were labeled with I-131 at high activity levels without excessive chemical damage. Up to 20 mg of Fab were labeled with up to 300 mCi of I-131 using the chloramine-T method and large working volumes at room temperature. As much as 90% of the initial activity was recovered as labeled product. The labeled Fabs varied in their sensitivity to radioiodination damage, as measured by an in vitro cell-binding assay. Radioionidation was performed safely using a remote iodination apparatus. The final product was of radiopharmaceutical quality suitable for clinical diagnosis and experimental radiotherapy in humans.

Original languageEnglish (US)
Pages (from-to)367-370
Number of pages4
JournalJournal of Nuclear Medicine
Volume25
Issue number3
StatePublished - May 3 1984

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Ferens, J. M., Krohn, K. A., Beaumier, P. L., Brown, J. P., Hellström, I., Hellström, K. E., Carrasquillo, J. A., & Larson, S. M. (1984). High-level iodination of monoclonal antibody fragments for radiotherapy. Journal of Nuclear Medicine, 25(3), 367-370.