High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates

Cecilia Cheung, Victoria Roberts, Antonio Frias, Robert A. Brace

Research output: Contribution to journalArticle

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Abstract

Western style, high-fat diet (HFD) and associated high lipid levels have deleterious effects on fetal and placental development independent of maternal obesity and/or diabetes. Our objectives were to determine whether HFD without development of obesity would alter amniotic fluid volume (AFV) and amnion aquaporin (AQP) expression in a non-human primate model. Japanese macaques were fed either a control diet or HFD before and during pregnancy. The four quadrant amniotic fluid index (AFI) was used as an ultrasonic estimate of AFV at 120 days gestation. Amnion samples were collected at 130 days gestation by cesarean section and AQP mRNA levels were determined by quantitative RT-PCR. Similar to that in human, AQP1, AQP3, AQP8, AQP9, and AQP11 were expressed in the macaque amnion with significant differences in levels among AQPs. In macaque, neither individual AQPs nor expression profiles of the five AQPs differed between control and non-obese HFD animals. There were regional differences in AQP expression in that, AQP1 mRNA levels were highest and AQP8 lowest in reflected amnion while AQP3, AQP9, and AQP11 were not different among amnion regions. When subdivided into control and HFD groups, AQP1 mRNA levels remain highest in the reflected amnion of both groups. The HFD did not significantly affect the AFI, but AFI was positively correlated with AQP11 mRNA levels independent of diet. Collectively, these data suggest that HFD in pregnant non-obese individuals may have at most modest effects on AFV as the AFI and amnion AQP expression are not substantially altered.

Original languageEnglish (US)
Article numbere13792
JournalPhysiological Reports
Volume6
Issue number14
DOIs
StatePublished - Jul 1 2018

Fingerprint

Aquaporins
Amnion
High Fat Diet
Amniotic Fluid
Primates
Macaca
Messenger RNA
Pregnancy
Obesity
Diet
Placentation
Fetal Development
Ultrasonics
Cesarean Section
Mothers
Lipids
Polymerase Chain Reaction

Keywords

  • Amniotic fluid volume
  • aquaporins
  • high-fat diet
  • monkey

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates. / Cheung, Cecilia; Roberts, Victoria; Frias, Antonio; Brace, Robert A.

In: Physiological Reports, Vol. 6, No. 14, e13792, 01.07.2018.

Research output: Contribution to journalArticle

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