High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets

Cynthia Bethea, Arubala Reddy, Matthew Flowers, Robert A. Shapiro, Ricki J. Colman, David H. Abbott, Jon E. Levine

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The administration of estradiol-17β (E) to animal models after loss of ovarian steroid production has many beneficial effects on neural functions, particularly in the serotonin system in nonhuman primates (NHPs). E also has anorexic effects, although the mechanism of action is not well defined. In the US, obesity has reached epidemic proportions, and blame is partially directed at the Western style diet, which is high in fat and sugar. This study examined the interaction of E and diet in surgically menopausal nonhuman primates with a 2. ×. 2 block design. Marmosets (. Callithrix jacchus; n=. 4/group) were placed on control-low fat diet (LFD; 14%kcal from fat) or high fat diet (HFD; 28%kcal from fat) 1. month prior to ovariectomy (Ovx). Empty (placebo) or E-filled Silastic capsules were implanted immediately following Ovx surgery. Treatments extended 6. months. The established groups were: placebo. +. LFD, E. +. LFD, placebo. +. HFD, or E. +. HFD. At necropsy, the brain was flushed with saline and harvested. The midbrain was dissected and a small block containing the dorsal raphe nucleus was processed for qRT-PCR using Evagreen (Biotinum). Genes previously found to impact serotonin neural functions were examined. Results were compared with 2-way ANOVA followed by Bonferroni post-hoc tests or Cohen's D analysis. There was a significant effect of treatment on tryptophan hydroxylase 2 (TPH2) across the groups (. p=. 0.019). E stimulated TPH2 expression and HFD prevented E-stimulated TPH2 expression (. p

    Original languageEnglish (US)
    Pages (from-to)71-80
    Number of pages10
    JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
    Volume58
    DOIs
    StatePublished - Apr 3 2015

    Fingerprint

    Tryptophan Hydroxylase
    Callithrix
    High Fat Diet
    Serotonin
    Estrogens
    Fats
    Placebos
    Gene Expression
    Primates
    Diet
    Fat-Restricted Diet
    Ovariectomy
    Mesencephalon
    Capsules
    Estradiol
    Analysis of Variance
    Animal Models
    Obesity
    Steroids
    Polymerase Chain Reaction

    Keywords

    • Estrogen
    • Non-human primate
    • Serotonin
    • Use Diet

    ASJC Scopus subject areas

    • Biological Psychiatry
    • Pharmacology

    Cite this

    High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets. / Bethea, Cynthia; Reddy, Arubala; Flowers, Matthew; Shapiro, Robert A.; Colman, Ricki J.; Abbott, David H.; Levine, Jon E.

    In: Progress in Neuro-Psychopharmacology and Biological Psychiatry, Vol. 58, 03.04.2015, p. 71-80.

    Research output: Contribution to journalArticle

    Bethea, Cynthia ; Reddy, Arubala ; Flowers, Matthew ; Shapiro, Robert A. ; Colman, Ricki J. ; Abbott, David H. ; Levine, Jon E. / High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets. In: Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2015 ; Vol. 58. pp. 71-80.
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    abstract = "The administration of estradiol-17β (E) to animal models after loss of ovarian steroid production has many beneficial effects on neural functions, particularly in the serotonin system in nonhuman primates (NHPs). E also has anorexic effects, although the mechanism of action is not well defined. In the US, obesity has reached epidemic proportions, and blame is partially directed at the Western style diet, which is high in fat and sugar. This study examined the interaction of E and diet in surgically menopausal nonhuman primates with a 2. ×. 2 block design. Marmosets (. Callithrix jacchus; n=. 4/group) were placed on control-low fat diet (LFD; 14{\%}kcal from fat) or high fat diet (HFD; 28{\%}kcal from fat) 1. month prior to ovariectomy (Ovx). Empty (placebo) or E-filled Silastic capsules were implanted immediately following Ovx surgery. Treatments extended 6. months. The established groups were: placebo. +. LFD, E. +. LFD, placebo. +. HFD, or E. +. HFD. At necropsy, the brain was flushed with saline and harvested. The midbrain was dissected and a small block containing the dorsal raphe nucleus was processed for qRT-PCR using Evagreen (Biotinum). Genes previously found to impact serotonin neural functions were examined. Results were compared with 2-way ANOVA followed by Bonferroni post-hoc tests or Cohen's D analysis. There was a significant effect of treatment on tryptophan hydroxylase 2 (TPH2) across the groups (. p=. 0.019). E stimulated TPH2 expression and HFD prevented E-stimulated TPH2 expression (. p",
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