High efficiency plating method for Leishmania promastigotes in semidefined or completely-defined medium

D. M. Iovannisci, Buddy Ullman

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

A simple technique, developed for the isolation of clones derived from single, promastigote cells of Leishmania donovani and Leishmania tropica, involved the use of semisolid agar. Both species of Leishmania promastigotes formed discrete colonies at high efficiency either in semidefined medium containing 10% fetal calf serum or in completely-defined medium lacking serum. Visible colonies appeared between 8 and 14 days in growth medium containing 10% fetal calf serum. Replacement of the fetal calf serum with bovine serum albumin and Tween-80 increased the time of colony formation by 50% but did not affect the cloning efficiency. Viability of colonies transferred from semisolid agar to liquid suspension culture was 100%.

Original languageEnglish (US)
Pages (from-to)633-636
Number of pages4
JournalJournal of Parasitology
Volume69
Issue number4
StatePublished - 1983
Externally publishedYes

Fingerprint

promastigotes
Leishmania
fetal bovine serum
serum
Leishmania tropica
agar
Serum
Agar
Leishmania donovani
bovine serum albumin
blood serum
Polysorbates
molecular cloning
Bovine Serum Albumin
culture media
methodology
viability
clones
Organism Cloning
Suspensions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology
  • Parasitology

Cite this

High efficiency plating method for Leishmania promastigotes in semidefined or completely-defined medium. / Iovannisci, D. M.; Ullman, Buddy.

In: Journal of Parasitology, Vol. 69, No. 4, 1983, p. 633-636.

Research output: Contribution to journalArticle

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