High content cellular analysis for functional screening of novel cell cycle related genes

Desok Kim, Yong Su Chae, Soo Jung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Functional screening of novel genes can be efficiently performed by detecting morphological changes of transfected cells. In this study, we developed automated cellular analysis software and found novel genes that induced mitotic phenotypes in transiently transfected HeLa cells. Systemic imaging errors common in high content microscopy were corrected by background subtraction and intensity normalization. Nuclear and chromatin objects were detected by tophat operations. Shape, intensity, and convex-hull features were extracted. Cell types were classified by several logistic regression formulas with 93.9% accuracy in average. The increased % mitotic cells were detected in 47 out of 571 transfected genes. Twenty genes showed more than two-fold increase in % mitotic cells confirmed by manual inspection. Among these, nine genes showed increased tissue expression levels in several tumors, thus, indicating their possible oncogenic roles. These genes will be further investigated biochemically to confirm their cell cycle related functions.

Original languageEnglish (US)
Title of host publicationBioMedical Engineering and Informatics
Subtitle of host publicationNew Development and the Future - Proceedings of the 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008
Pages148-152
Number of pages5
DOIs
StatePublished - Sep 17 2008
EventBioMedical Engineering and Informatics: New Development and the Future - 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008 - Sanya, Hainan, China
Duration: May 27 2008May 30 2008

Publication series

NameBioMedical Engineering and Informatics: New Development and the Future - Proceedings of the 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008
Volume1

Other

OtherBioMedical Engineering and Informatics: New Development and the Future - 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008
CountryChina
CitySanya, Hainan
Period5/27/085/30/08

ASJC Scopus subject areas

  • Information Systems
  • Signal Processing
  • Biomedical Engineering

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  • Cite this

    Kim, D., Chae, Y. S., & Kim, S. J. (2008). High content cellular analysis for functional screening of novel cell cycle related genes. In BioMedical Engineering and Informatics: New Development and the Future - Proceedings of the 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008 (pp. 148-152). [4548653] (BioMedical Engineering and Informatics: New Development and the Future - Proceedings of the 1st International Conference on BioMedical Engineering and Informatics, BMEI 2008; Vol. 1). https://doi.org/10.1109/BMEI.2008.233