High-concentration rapid transients of glutamate mediate neural-glial communication via ectopic release

Ko Matsui, Craig E. Jahr, Maria E. Rubio

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

Until recently, communication from neurons to astrocytes was thought to be mediated by low-concentration transients of glutamate caused by spillover from the synaptic cleft. However, quantal events recorded in rat cerebellar Bergmann glial cells (BGs) have fast kinetics, comparable with those recorded in neurons. By combining outside-out patch recordings of BG AMPA receptors and quantitative electron microscopic analysis of glutamate receptor subunit 1 (GluR1) and GluR4 immunogold labeling measurements, at both the soma and membranes surrounding synapses, we estimate the absolute density of functional AMPA receptors. Using a kinetic model of BG AMPA receptors, we find that quantal events recorded in BGs are produced by high-concentration (∼1-1.5 mM), fast transients (∼0.5 ms decay) of glutamate, similar to transients within the synaptic cleft. Our results indicate that neural signaling to BGs is mediated by ectopic release of transmitter from presynaptic elements directly facing the BG membrane.

Original languageEnglish (US)
Pages (from-to)7538-7547
Number of pages10
JournalJournal of Neuroscience
Volume25
Issue number33
DOIs
StatePublished - Aug 17 2005

Keywords

  • Bergmann glial cell
  • Ectopic release
  • Glutamate
  • Immunogold electron microscopy
  • Purkinje cell
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

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