Hierarchies of protein cross-linking in the extracellular matrix

Involvement of an egg surface transglutaminase in early stages of fertilization envelope assembly

David Battaglia, N. M. Shapiro

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The involvement of transglutaminase activity in fertilization envelope (FE) formation was investigated using eggs from the sea urchin, Strongylocentrotus purpuratus. Eggs fertilized in the presence of the transglutaminase inhibitors, putrescine and cadaverine, had disorganized and expanded FEs with inhibition of the characteristic I-T transition. The permeability of the FE was increased by these agents, as revealed by the loss of proteins from the perivitelline space and the appearance of ovoperoxidase activity in supernates from putrescine-treated eggs. [3H]putrescine was incorporated into the FE during fertilization in a reaction catalyzed by an egg surface transglutaminase that could also use dimethylcasein as a substrate in vitelline layer-denuded eggs. Egg secretory products alone had no transglutaminase activity. The cell surface transglutaminase activity was transient and maximal within 4 min of activation. The enzyme was Ca2+ dependent and was inhibited by Zn2+. We conclude that sea urchin egg surface transglutaminase catalyzes an early step in a hierarchy of cross-linking events during FE assembly, one that occurs before ovoperoxidase-mediated dityrosine formation(Foerder, C.A., and B.M. Shapiro. 1977. Proc. Natl. Acad. Sci. USA. 74:4214-4218). Thus it provides a graphic example of the physiological function of a cell surface transglutaminase.

Original languageEnglish (US)
Pages (from-to)2447-2454
Number of pages8
JournalJournal of Cell Biology
Volume107
Issue number6 I
StatePublished - 1988
Externally publishedYes

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Transglutaminases
Fertilization
Extracellular Matrix
Ovum
Putrescine
Proteins
Eggs
Peroxidases
Sea Urchins
Strongylocentrotus purpuratus
Cadaverine
Zygote
Permeability
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hierarchies of protein cross-linking in the extracellular matrix : Involvement of an egg surface transglutaminase in early stages of fertilization envelope assembly. / Battaglia, David; Shapiro, N. M.

In: Journal of Cell Biology, Vol. 107, No. 6 I, 1988, p. 2447-2454.

Research output: Contribution to journalArticle

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