Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system

Matthias Schaefer, Mark Petronczki, Daniela Dorner, Michael Forte, Juergen A. Knoblich

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

In Drosophila, distinct mechanisms orient asymmetric cell division along the apical-basal axis in neuroblasts and along the anterior-posterior axis in sensory organ precursor (SOP) cells. Here, we show that heterotrimeric G proteins are essential for asymmetric cell division in both cell types. The G protein subunit Gαi localizes apically in neuroblasts and anteriorly in SOP cells before and during mitosis. Interfering with G protein function by Gαi overexpression or depletion of heterotrimeric G protein complexes causes defects in spindle orientation and asymmetric localization of determinants. Gαi is colocalized and associated with Pins, a protein that induces the release of the βγ subunit and might act as a receptor-independent G protein activator. Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types.

Original languageEnglish (US)
Pages (from-to)183-194
Number of pages12
JournalCell
Volume107
Issue number2
DOIs
StatePublished - Oct 19 2001

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Asymmetric Cell Division
Heterotrimeric GTP-Binding Proteins
Neurology
Nervous System
Drosophila
Cells
GTP-Binding Proteins
Gi-Go GTP-Binding Protein alpha Subunits
Chemical activation
Mitosis
Defects
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system. / Schaefer, Matthias; Petronczki, Mark; Dorner, Daniela; Forte, Michael; Knoblich, Juergen A.

In: Cell, Vol. 107, No. 2, 19.10.2001, p. 183-194.

Research output: Contribution to journalArticle

Schaefer, Matthias ; Petronczki, Mark ; Dorner, Daniela ; Forte, Michael ; Knoblich, Juergen A. / Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system. In: Cell. 2001 ; Vol. 107, No. 2. pp. 183-194.
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