Heteropolymeric potassium channels expressed in xenopus oocytes from cloned subunits

M. J. Christie, R. A. North, P. B. Osborne, J. Douglass, J. P. Adelman

Research output: Contribution to journalArticle

204 Scopus citations

Abstract

Voltage-dependent potassium currents were measured in Xenopus oocytes previously injected with RNAs generated in vitro from each of three cloned cDNAs (RBK1, RBK2, and RGK5). The currents differed in their sensitivities to blockade by tetraethylammonium (TEA; respective KDS 0.3, >100, and 10 mM) and in their inactivation during a depolarizing pulse. Injections of RNA combinations (RBK1/RBK2 and RBK1/RGK5) caused currents that had TEA sensitivities different from those expected from the sum, in any proportion, of the two native channels. It is concluded that novel potassium channels are formed by the oocytes injected with two RNAs, presumably by heteropolymerization of subunits; such heteropolymerization would contribute functional diversity to voltage-dependent potassium channels in addition to that provided by a large gene family.

Original languageEnglish (US)
Pages (from-to)405-411
Number of pages7
JournalNeuron
Volume4
Issue number3
DOIs
StatePublished - Mar 1990

ASJC Scopus subject areas

  • Neuroscience(all)

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    Christie, M. J., North, R. A., Osborne, P. B., Douglass, J., & Adelman, J. P. (1990). Heteropolymeric potassium channels expressed in xenopus oocytes from cloned subunits. Neuron, 4(3), 405-411. https://doi.org/10.1016/0896-6273(90)90052-H