Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men

Huifen Li, Joseph B. Margolick, Jay H. Bream, Tricia L. Nilles, Susan Langan, Hanhvy T. Bui, Andrew W. Sylwester, Louis J. Picker, Sean X. Leng

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed <12% and <40% of the total CD4+ and CD8+ T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.

Original languageEnglish (US)
Pages (from-to)400-404
Number of pages5
JournalJournal of Infectious Diseases
Volume210
Issue number3
DOIs
StatePublished - Aug 1 2014

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Keywords

  • CMV
  • HIV infection
  • IE-1
  • IFN-γ
  • IL-2
  • Intracellular cytokine staining
  • MACS
  • Pp65
  • TNF-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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