Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men

Huifen Li, Joseph B. Margolick, Jay H. Bream, Tricia L. Nilles, Susan Langan, Hanhvy T. Bui, Andrew Sylwester, Louis Picker, Sean X. Leng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed + and CD8+ T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.

Original languageEnglish (US)
Pages (from-to)400-404
Number of pages5
JournalJournal of Infectious Diseases
Volume210
Issue number3
DOIs
StatePublished - Aug 1 2014

Fingerprint

Cytomegalovirus
HIV
T-Lymphocytes
Antigens
Open Reading Frames
HIV-1
Immunity
Tissue Donors
Cytokines
Peptides

Keywords

  • CMV
  • HIV infection
  • IE-1
  • IFN-γ
  • IL-2
  • Intracellular cytokine staining
  • MACS
  • Pp65
  • TNF-α

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men. / Li, Huifen; Margolick, Joseph B.; Bream, Jay H.; Nilles, Tricia L.; Langan, Susan; Bui, Hanhvy T.; Sylwester, Andrew; Picker, Louis; Leng, Sean X.

In: Journal of Infectious Diseases, Vol. 210, No. 3, 01.08.2014, p. 400-404.

Research output: Contribution to journalArticle

Li, Huifen ; Margolick, Joseph B. ; Bream, Jay H. ; Nilles, Tricia L. ; Langan, Susan ; Bui, Hanhvy T. ; Sylwester, Andrew ; Picker, Louis ; Leng, Sean X. / Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men. In: Journal of Infectious Diseases. 2014 ; Vol. 210, No. 3. pp. 400-404.
@article{31ce4a13a8d840909a7246e4b3e743d5,
title = "Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men",
abstract = "Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed + and CD8+ T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.",
keywords = "CMV, HIV infection, IE-1, IFN-γ, IL-2, Intracellular cytokine staining, MACS, Pp65, TNF-α",
author = "Huifen Li and Margolick, {Joseph B.} and Bream, {Jay H.} and Nilles, {Tricia L.} and Susan Langan and Bui, {Hanhvy T.} and Andrew Sylwester and Louis Picker and Leng, {Sean X.}",
year = "2014",
month = "8",
day = "1",
doi = "10.1093/infdis/jiu093",
language = "English (US)",
volume = "210",
pages = "400--404",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Heterogeneity of CD4+ and CD8+ T-cell responses to cytomegalovirus in HIV-infected and HIV-uninfected men who have sex with men

AU - Li, Huifen

AU - Margolick, Joseph B.

AU - Bream, Jay H.

AU - Nilles, Tricia L.

AU - Langan, Susan

AU - Bui, Hanhvy T.

AU - Sylwester, Andrew

AU - Picker, Louis

AU - Leng, Sean X.

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed + and CD8+ T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.

AB - Studies of T-cell immunity to human cytomegalovirus (CMV) primarily reflect anti-CMV pp65 or immediate early antigen 1 (IE-1) activity. We assessed responses of T cells from human immunodeficiency virus (HIV)-negative and HIV-infected men to peptide pools spanning 19 CMV open reading frames selected because they previously correlated with total CMV-specific T-cell responses in healthy donors. Cells producing cytokines in response to pp65 or IE-1 together composed + and CD8+ T-cell responses to CMV, respectively. These proportions were generally similar regardless of HIV serostatus. Thus, analyses of total CMV-specific T-cell responses should extend beyond pp65 and IE-1 regardless of HIV serostatus.

KW - CMV

KW - HIV infection

KW - IE-1

KW - IFN-γ

KW - IL-2

KW - Intracellular cytokine staining

KW - MACS

KW - Pp65

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=84904298047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904298047&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiu093

DO - 10.1093/infdis/jiu093

M3 - Article

C2 - 24532602

AN - SCOPUS:84904298047

VL - 210

SP - 400

EP - 404

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 3

ER -