Herpesviruses in Abscesses and Cellulitis of Endodontic Origin

Vicky Chen, Yanwen Chen, Hong Li, Karla Kent, John (Craig) Baumgartner, Curtis Machida

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Acute apical abscesses and cellulitis are severe endodontic diseases caused by opportunistic bacteria with possible coinfection with latent herpesviruses. The objectives of this study are to identify herpesviruses, including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), herpes simplex virus-1 (HSV-1), and Varicella zoster virus (VZV) in patients (n = 31) presenting with acute apical abscesses and cellulitis of endodontic origin. Primary and nested polymerase chain reaction (PCR) was conducted using virus-specific primers and DNA isolated from cell-free abscess fluid. From patients exhibiting concurrent spontaneous pain (n = 28), nine abscesses contained HCMV, two abscesses contained EBV, one abscess contained HSV-1, and no abscesses contained VZV. Control PCR using genomic or recombinant templates showed detection limits to a single genomic copy of HCMV, 100 genomic copies for EBV, and 1 to 10 copies for HSV-1 with no cross-amplification between herpesviral DNA targets. Nested PCR was required for detection of herpesviral DNA in the abscess specimens, indicating that these viruses were present in low copy number. Filtration of abscess specimens and virus transfer experiments using human fibroblastic MRC-5 cells confirmed the presence of HCMV particles in several abscess specimens. We conclude that herpesviruses are present but not required for the development of acute apical abscesses and cellulitis of endodontic origin.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalJournal of Endodontics
Volume35
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

Cellulitis
Herpesviridae
Endodontics
Abscess
Cytomegalovirus
Human Herpesvirus 1
Human Herpesvirus 4
Human Herpesvirus 3
Viruses
Polymerase Chain Reaction
DNA Primers
DNA
Coinfection
Limit of Detection
Bacteria

Keywords

  • Acute apical abscess
  • apical periodontitis
  • cellulitis
  • endodontic infections
  • Epstein-Barr virus
  • herpes simplex virus
  • herpesviruses
  • human cytomegalovirus
  • Varicella zoster virus

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Herpesviruses in Abscesses and Cellulitis of Endodontic Origin. / Chen, Vicky; Chen, Yanwen; Li, Hong; Kent, Karla; Baumgartner, John (Craig); Machida, Curtis.

In: Journal of Endodontics, Vol. 35, No. 2, 02.2009, p. 182-188.

Research output: Contribution to journalArticle

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