Herpes simplex virus type 2 ICP47 inhibits human TAP but not mouse tap

R. Tomazin, N. E.G. Van Schoot, K. Goldsmith, P. Jugovic, P. Sempe, K. Fruh, D. C. Johnson

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Herpes simplex virus serotype 1 (HSV-1) expresses an immediate-early protein, ICP47, that effectively blocks the major histocompatibility complex class I antigen presentation pathway. HSV-1 ICP47 (ICP47-1) binds with high affinity to the human transporter associated with antigen presentation (TAP) and blocks the binding of antigenic peptides. HSV type 2 (HSV-2) ICP47 (ICP47-2) has only 42% amino acid sequence identity with ICP47-1. Here, we compared the levels of inhibition of human and murine TAP, expressed in insect cell microsomes, by ICP47-1 and ICP47-2. Both proteins inhibited human TAP at similar concentrations, and the K(D) for ICP47-2 binding to human TAP was 4.8 x 10-8 M, virtually identical to that measured for ICP47-1 (5.2 x 10-8 M). There was some inhibition of murine TAP by both ICP47-2 and ICP47- 1, but this inhibition was incomplete and only at ICP47 concentrations 50 to 100 times that required to inhibit human TAP. Lack of inhibition of murine TAP by ICP47-1 and ICP47-2 could be explained by an inability of both proteins to bind to murine TAP.

Original languageEnglish (US)
Pages (from-to)2560-2563
Number of pages4
JournalJournal of virology
Volume72
Issue number3
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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