Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm

Aaron Farnsworth, Kimberly Goldsmith, David Johnson

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The late stages of assembly of herpes simplex virus (HSV) and other herpesviruses are not well understood. Acquisition of the final virion envelope apparently involves interactions between viral nucleocapsids coated with tegument proteins and the cytoplasmic domains of membrane glycoproteins. This promotes budding of virus particles into cytoplasmic vesicles derived from the trans-Golgi network or endosomes. The identities of viral membrane glycoproteins and tegument proteins involved in these processes are not well known. Here, we report that HSV mutants lacking two viral glycoproteins, gD and gE, accumulated large numbers of unenveloped nucleocapsids in the cytoplasm. These aggregated capsids were immersed in an electron-dense layer that appeared to be tegument. Few or no enveloped virions were observed. More subtle defects were observed with an HSV unable to express gD and gI. A triple mutant lacking gD, gE, and gI exhibited more severe defects in envelopment. We concluded that HSV gD and the gE/gI heterodimeric complex act in a redundant fashion to anchor the virion envelope onto tegument-coated capsids. In the absence of either one of these HSV glycoproteins, envelopment proceeds; however, without both gD and gE, or gE/gI, there is profound inhibition of cytoplasmic envelopment.

Original languageEnglish (US)
Pages (from-to)8481-8494
Number of pages14
JournalJournal of Virology
Volume77
Issue number15
DOIs
StatePublished - Aug 2003

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herpes simplex
Simplexvirus
virion
Virion
glycoproteins
Glycoproteins
Cytoplasm
cytoplasm
viruses
membrane glycoproteins
Nucleocapsid
nucleocapsid
capsid
Capsid
Membrane Glycoproteins
Cytoplasmic Vesicles
trans-Golgi Network
mutants
endosomes
Herpesviridae

ASJC Scopus subject areas

  • Immunology

Cite this

Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm. / Farnsworth, Aaron; Goldsmith, Kimberly; Johnson, David.

In: Journal of Virology, Vol. 77, No. 15, 08.2003, p. 8481-8494.

Research output: Contribution to journalArticle

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