Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane

Aaron Farnsworth, Todd W. Wisner, Michael Webb, Richard Roller, Gary Cohen, Roselyn Eisenberg, David Johnson

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Herpesviruses must traverse the nuclear envelope to gain access to the cytoplasm and, ultimately, to exit cells. It is believed that herpesvirus nucleocapsids enter the perinuclear space by budding through the inner nuclear membrane (NM). To reach the cytoplasm these enveloped particles must fuse with the outer NM and the unenveloped capsids then acquire a second envelope in the trans-Golgi network. Little is known about the process by which herpesviruses virions fuse with the outer NM. Here we show that a herpes simplex virus (HSV) mutant lacking both the two putative fusion glycoproteins gB and gH failed to cross the nuclear envelope. Enveloped virions accumulated in the perinuclear space or in membrane vesicles that bulged into the nucleoplasm (herniations). By contrast, mutants lacking just gB or gH showed only minor or no defects in nuclear egress. We concluded that either HSV gB or gH can promote fusion between the virion envelope and the outer NM. It is noteworthy that fusion associated with HSV entry requires the cooperative action of both gB and gH, suggesting that the two types of fusion (egress versus entry) are dissimilar processes.

Original languageEnglish (US)
Pages (from-to)10187-10192
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number24
DOIs
StatePublished - Jun 12 2007

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Nuclear Envelope
Simplexvirus
Virion
Glycoproteins
Herpesviridae
Cytoplasm
trans-Golgi Network
Nucleocapsid
Virus Internalization
Capsid
Membranes

Keywords

  • Deenvelopment
  • Egress
  • Nuclear envelope
  • Perinuclear space

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane. / Farnsworth, Aaron; Wisner, Todd W.; Webb, Michael; Roller, Richard; Cohen, Gary; Eisenberg, Roselyn; Johnson, David.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 24, 12.06.2007, p. 10187-10192.

Research output: Contribution to journalArticle

Farnsworth, Aaron ; Wisner, Todd W. ; Webb, Michael ; Roller, Richard ; Cohen, Gary ; Eisenberg, Roselyn ; Johnson, David. / Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 24. pp. 10187-10192.
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