Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors

Craig R. Brunetti, Rae Lyn Burke, Stuart Kornfeld, Walter Gregory, Frank R. Masiarz, Kevin S. Dingwell, David Johnson

Research output: Contribution to journalArticle

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Abstract

Herpes simplex viruses (HSV) use multiple and sequential receptors to enter host cells. HSV glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. We used soluble forms of gD that were expressed in Chinese hamster ovary cells to characterize and identify a putative cellular receptor for HSV as the 275- kDa mannose 6-phosphate/insulin-like growth factor II receptor. Soluble gD also bound to the 46-kDa cation-dependent mannose 6-phosphate (Man-6-P) receptor and was extensively modified with Man-6-P residues on its Asn- linked oligosaccharides. Additionally, soluble gD was a high affinity substrate for N-acetylglucosamine-1-phosphotransferase, the first enzyme in the biosynthetic pathway for the addition of Man-6-P residues to lysosomal enzymes. The membrane form of gD immunoprecipitated from HSV-infected cells also bound to the 275-kDa mannose 6-phosphate/insulin-like growth factor II receptor, albeit poorly and only a small fraction of the membrane gD was modified with Man-6-P. Notwithstanding this low level of mannose phosphorylation, the interaction between gD and Man-6-P receptors may play a role in some aspect of virus entry or egress.

Original languageEnglish (US)
Pages (from-to)17067-17074
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number25
StatePublished - Jun 24 1994
Externally publishedYes

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IGF Type 2 Receptor
Simplexvirus
Viruses
Glycoproteins
Membrane Glycoproteins
Mannose
Virus Release
Virus Receptors
Virus Internalization
Biosynthetic Pathways
Enzymes
Cricetulus
Phosphorylation
Oligosaccharides
Ovary
mannose-6-phosphate
Cells
Membranes
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Brunetti, C. R., Burke, R. L., Kornfeld, S., Gregory, W., Masiarz, F. R., Dingwell, K. S., & Johnson, D. (1994). Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors. Journal of Biological Chemistry, 269(25), 17067-17074.

Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors. / Brunetti, Craig R.; Burke, Rae Lyn; Kornfeld, Stuart; Gregory, Walter; Masiarz, Frank R.; Dingwell, Kevin S.; Johnson, David.

In: Journal of Biological Chemistry, Vol. 269, No. 25, 24.06.1994, p. 17067-17074.

Research output: Contribution to journalArticle

Brunetti, CR, Burke, RL, Kornfeld, S, Gregory, W, Masiarz, FR, Dingwell, KS & Johnson, D 1994, 'Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors', Journal of Biological Chemistry, vol. 269, no. 25, pp. 17067-17074.
Brunetti CR, Burke RL, Kornfeld S, Gregory W, Masiarz FR, Dingwell KS et al. Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors. Journal of Biological Chemistry. 1994 Jun 24;269(25):17067-17074.
Brunetti, Craig R. ; Burke, Rae Lyn ; Kornfeld, Stuart ; Gregory, Walter ; Masiarz, Frank R. ; Dingwell, Kevin S. ; Johnson, David. / Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 25. pp. 17067-17074.
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