Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors

Craig R. Brunetti, Rae Lyn Burke, Stuart Kornfeld, Walter Gregory, Frank R. Masiarz, Kevin S. Dingwell, David C. Johnson

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Herpes simplex viruses (HSV) use multiple and sequential receptors to enter host cells. HSV glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. We used soluble forms of gD that were expressed in Chinese hamster ovary cells to characterize and identify a putative cellular receptor for HSV as the 275- kDa mannose 6-phosphate/insulin-like growth factor II receptor. Soluble gD also bound to the 46-kDa cation-dependent mannose 6-phosphate (Man-6-P) receptor and was extensively modified with Man-6-P residues on its Asn- linked oligosaccharides. Additionally, soluble gD was a high affinity substrate for N-acetylglucosamine-1-phosphotransferase, the first enzyme in the biosynthetic pathway for the addition of Man-6-P residues to lysosomal enzymes. The membrane form of gD immunoprecipitated from HSV-infected cells also bound to the 275-kDa mannose 6-phosphate/insulin-like growth factor II receptor, albeit poorly and only a small fraction of the membrane gD was modified with Man-6-P. Notwithstanding this low level of mannose phosphorylation, the interaction between gD and Man-6-P receptors may play a role in some aspect of virus entry or egress.

Original languageEnglish (US)
Pages (from-to)17067-17074
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number25
StatePublished - Jun 24 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Brunetti, C. R., Burke, R. L., Kornfeld, S., Gregory, W., Masiarz, F. R., Dingwell, K. S., & Johnson, D. C. (1994). Herpes simplex virus glycoprotein D acquires mannose 6-phosphate residues and binds to mannose 6-phosphate receptors. Journal of Biological Chemistry, 269(25), 17067-17074.