Herpes simplex virus gE/gI extracellular domains promote axonal transport and spread from neurons to epithelial cells

Paul W. Howard, Catherine C. Wright, Tiffani Howard, David Johnson

Research output: Contribution to journalArticle

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Abstract

Following reactivation from latency, there are two distinct steps in the spread of herpes simplex virus (HSV) from infected neurons to epithelial cells: (i) anterograde axonal transport of virus particles from neuron bodies to axon tips and (ii) exocytosis and spread of extracellular virions across cell junctions into adjacent epithelial cells. The HSV heterodimeric glycoprotein gE/gI is important for anterograde axonal transport, and gE/gI cytoplasmic domains play important roles in sorting of virus particles into axons. However, the roles of the large (&tild; 400-residue) gE/gI extracellular (ET) domains in both axonal transport and neuron-to-epithelial cell spread have not been characterized. Two gE mutants, gE-277 and gE-348, contain small insertions in the gE ET domain, fold normally, form gE/gI heterodimers, and are incorporated into virions. Both gE-277 and gE-348 did not function in anterograde axonal transport; there were markedly reduced numbers of viral capsids and glycoproteins compared with wildtype HSV. The defects in axonal transport were manifest in neuronal cell bodies, involving missorting of HSV capsids before entry into proximal axons. Although there were diminished numbers of mutant gE-348 capsids and glycoproteins in distal axons, there was efficient spread to adjacent epithelial cells, similar to wild-type HSV. In contrast, virus particles produced by HSV gE-277 spread poorly to epithelial cells, despite numbers of virus particles similar to those for HSV gE-348. These results genetically separate the two steps in HSV spread from neurons to epithelial cells and demonstrate that the gE/gI ET domains function in both processes.

Original languageEnglish (US)
Pages (from-to)11178-11186
Number of pages9
JournalJournal of Virology
Volume88
Issue number19
DOIs
StatePublished - 2014

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herpes simplex
Axonal Transport
Simplexvirus
epithelial cells
neurons
Epithelial Cells
virion
Virion
Neurons
viruses
axons
capsid
Axons
Capsid
glycoproteins
Glycoproteins
intercellular junctions
mutants
Intercellular Junctions
axonal transport

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Herpes simplex virus gE/gI extracellular domains promote axonal transport and spread from neurons to epithelial cells. / Howard, Paul W.; Wright, Catherine C.; Howard, Tiffani; Johnson, David.

In: Journal of Virology, Vol. 88, No. 19, 2014, p. 11178-11186.

Research output: Contribution to journalArticle

Howard, Paul W. ; Wright, Catherine C. ; Howard, Tiffani ; Johnson, David. / Herpes simplex virus gE/gI extracellular domains promote axonal transport and spread from neurons to epithelial cells. In: Journal of Virology. 2014 ; Vol. 88, No. 19. pp. 11178-11186.
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