Herpes simplex virus gE/gI and US9 promote both envelopment and sorting of virus particles in the cytoplasm of neurons, two processes that precede anterograde transport in axons

Grayson DuRaine, Todd W. Wisner, Paul Howard, Melissa Williams, David Johnson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Herpes simplex virus (HSV) anterograde transport in neuronal axons is vital, allowing spread from latently infected ganglia to epithelial tissues, where viral progeny are produced in numbers allowing spread to other hosts. The HSV membrane proteins gE/gI and US9 initiate the process of anterograde axonal transport, ensuring that virus particles are transported from the cytoplasm into the most proximal segments of axons. These proteins do not appear to be important once HSV is inside axons. We previously described HSV double mutants lacking both gE and US9 that failed to transport virus particles into axons. Here we show that gE- US9- double mutants accumulate large quantities of unenveloped and partially enveloped capsids in neuronal cytoplasm. These defects in envelopment can explain the defects in axonal transport of enveloped virions. In addition, the unenveloped capsids that accumulated were frequently bound to cytoplasmic membranes, apparently immobilized in intermediate stages of envelopment. A gE-null mutant produced enveloped virions, but these accumulated in large numbers in the neuronal cytoplasm rather than reaching cell surfaces as wild-type HSV virions do. Thus, in addition to the defects in envelopment, there was missorting of capsids and enveloped particles in the neuronal cytoplasm, which can explain the reduced anterograde transport of unenveloped capsids and enveloped virions. These mechanisms differ substantially from existing models suggesting that gE/gI and US9 function by tethering HSV particles to kinesin microtubule motors. The defects in assembly of gE- US9- mutant virus particles were novel because they were neuron specific, in keeping with observations that US9 is neuron specific.

Original languageEnglish (US)
Article numbere00050-17
JournalJournal of Virology
Volume91
Issue number11
DOIs
StatePublished - Jun 1 2017

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herpes simplex
Simplexvirus
virion
axons
Virion
sorting
Axons
Cytoplasm
cytoplasm
neurons
Neurons
viruses
capsid
Capsid
mutants
Axonal Transport
Kinesin
kinesin
Microtubules
membrane proteins

Keywords

  • Anterograde
  • Axons
  • Envelopment
  • Glycoproteins
  • Kinesins
  • Neuron

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Herpes simplex virus gE/gI and US9 promote both envelopment and sorting of virus particles in the cytoplasm of neurons, two processes that precede anterograde transport in axons. / DuRaine, Grayson; Wisner, Todd W.; Howard, Paul; Williams, Melissa; Johnson, David.

In: Journal of Virology, Vol. 91, No. 11, e00050-17, 01.06.2017.

Research output: Contribution to journalArticle

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