Herpes simplex virus capsids are transported in neuronal axons without an envelope containing the viral glycoproteins

Aleksandra Snyder, Todd W. Wisner, David Johnson

Research output: Contribution to journalArticle

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Abstract

Electron micrographic studies of neuronal axons have produced contradictory conclusions on how alpha-herpesviruses are transported from neuron cell bodies to axon termini. Some reports have described unenveloped capsids transported on axonal microtubules with separate transport of viral glycoproteins within membrane vesicles. Others have observed enveloped virions in proximal and distal axons. We characterized transport of herpes simplex virus (HSV) in human and rat neurons by staining permeabilized neurons with capsid- and glycoprotein-specific antibodies. Deconvolution microscopy was used to view 200-nm sections of axons. HSV glycoproteins were very rarely associated with capsids (3 to 5%) and vice versa. Instances of glycoprotein/capsid overlap frequently involved nonconcentric puncta and regions of axons with dense viral protein concentrations. Similarly, HSV capsids expressing a VP26-green fluorescent protein fusion protein (VP26/GFP) did not stain with antiglycoprotein antibodies. Live-cell imaging experiments with VP26/GFP-labeled capsids demonstrated that capsids moved in a saltatory fashion, and very few stalled for more than 1 to 2 min. To determine if capsids could be transported down axons without glycoproteins, neurons were treated with brefeldin A (BFA). However, BFA blocked both capsid and glycoprotein transport. Glycoproteins were transported into and down axons normally when neurons were infected with an HSV mutant that produces immature capsids that are retained in the nucleus. We concluded that HSV capsids are transported in axons without an envelope containing viral glycoproteins, with glycoproteins transported separately and assembling with capsids at axon termini.

Original languageEnglish (US)
Pages (from-to)11165-11177
Number of pages13
JournalJournal of Virology
Volume80
Issue number22
DOIs
StatePublished - Nov 2006

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herpes simplex
capsid
Capsid
Simplexvirus
axons
Axons
glycoproteins
Glycoproteins
viruses
neurons
Neurons
brefeldin A
Brefeldin A
Green Fluorescent Proteins
green fluorescent protein
vertebrate viruses
antibodies
Antibodies
Herpesviridae
viral proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Herpes simplex virus capsids are transported in neuronal axons without an envelope containing the viral glycoproteins. / Snyder, Aleksandra; Wisner, Todd W.; Johnson, David.

In: Journal of Virology, Vol. 80, No. 22, 11.2006, p. 11165-11177.

Research output: Contribution to journalArticle

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