Herbicide/pesticide effects on intestinal epithelial growth

Stephanie B. Greenman, Michael Rutten, Wendy M. Fowler, Larry Scheffler, Linda A. Shortridge, Beth Brown, Brett Sheppard, Karen Deveney, Clifford Deveney, Donald Trunkey

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effects of some common herbicides and pesticides on the growth of normal intestinal and colonic epithelial cells. Preconfluent cultures of normal rat intestinal cells (IEC-6 cell line) and normal human colonic epithelial cells were treated with 0.05-50 μM doses of atrazine, diazinon, and endosulfan. After 3 days of treatment, the change in cell proliferation was quantified by cell counting or the MTT growth assay. Both intestinal and colonic epithelial cell cultures had increases in cell growth when treated with as little as 1.0 μM atrazine, diazinon, or endosulfan. The observed changes in both cultured intestinal and colonic cell growth rates were not due to the influence of the vehicle control dimethyl sulfoxide (DMSO). That is, the treatment of the cell cultures with concentrations of DMSO as high as 0.5% for 3 days resulted in no change in cell growth compared with untreated control cultures. A consistent observation with all three of the compounds was that the highest doses (50 μM) had the least 'proliferative potential' in stimulating either IEC-6 cell or human colonic epithelial cell growth. Within the concentration range used, none of the herbicides or pesticides caused a decrease in cell proliferation below that of the untreated control cultures. Overall, treatment of IEC-6 cell cultures with atrazine, diazinon, or endosulfan produced a biphasic growth response, whereas the same treatment in the human colonic epithelial cell cultures produced a more sustained level of growth over the same period. This culture system may provide the basis for an in vitro model to further study the cellular and molecular basis of the effects of herbicides and pesticides on intestinal epithelial proliferation.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalEnvironmental Research
Volume75
Issue number1
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Pesticide effects
Herbicides
Cell culture
Pesticides
herbicide
pesticide
Cell growth
diazinon
Diazinon
Endosulfan
endosulfan
Atrazine
Growth
atrazine
Epithelial Cells
Cell Culture Techniques
Cell proliferation
Dimethyl Sulfoxide
Cell Proliferation
growth response

ASJC Scopus subject areas

  • Environmental Science(all)

Cite this

Greenman, S. B., Rutten, M., Fowler, W. M., Scheffler, L., Shortridge, L. A., Brown, B., ... Trunkey, D. (1997). Herbicide/pesticide effects on intestinal epithelial growth. Environmental Research, 75(1), 85-93. https://doi.org/10.1006/enrs.1997.3766

Herbicide/pesticide effects on intestinal epithelial growth. / Greenman, Stephanie B.; Rutten, Michael; Fowler, Wendy M.; Scheffler, Larry; Shortridge, Linda A.; Brown, Beth; Sheppard, Brett; Deveney, Karen; Deveney, Clifford; Trunkey, Donald.

In: Environmental Research, Vol. 75, No. 1, 10.1997, p. 85-93.

Research output: Contribution to journalArticle

Greenman, SB, Rutten, M, Fowler, WM, Scheffler, L, Shortridge, LA, Brown, B, Sheppard, B, Deveney, K, Deveney, C & Trunkey, D 1997, 'Herbicide/pesticide effects on intestinal epithelial growth', Environmental Research, vol. 75, no. 1, pp. 85-93. https://doi.org/10.1006/enrs.1997.3766
Greenman SB, Rutten M, Fowler WM, Scheffler L, Shortridge LA, Brown B et al. Herbicide/pesticide effects on intestinal epithelial growth. Environmental Research. 1997 Oct;75(1):85-93. https://doi.org/10.1006/enrs.1997.3766
Greenman, Stephanie B. ; Rutten, Michael ; Fowler, Wendy M. ; Scheffler, Larry ; Shortridge, Linda A. ; Brown, Beth ; Sheppard, Brett ; Deveney, Karen ; Deveney, Clifford ; Trunkey, Donald. / Herbicide/pesticide effects on intestinal epithelial growth. In: Environmental Research. 1997 ; Vol. 75, No. 1. pp. 85-93.
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