Hepatocyte growth factor increases alveolar liquid clearance in anesthetized, ventilated rats

A. N. Chesnutt, F. Tibayan, H. G. Folkesson, R. J. Mason, K. Shannon, M. A. Matthay

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, we have explored the possible contribution of growth factors, such as TGF-a, in upregulating alveolar sodium and fluid transport (Faseb J 9: A568, 1995). In this study, we tested the hypothesis that hepatocyte growth factor (HGF) could also increase alveolar fluid clearance. HGF is a heparin-binding growth factor that plays an important role in tissue repair. Also, we have detected substantial quantities of HGF in plasma and edema fluid of patients with acute lung injury. Thus, HGF may be an important factor in the recovery from acute lung injury. We studied (lie in vim effects of HGF on alveolar epithelial fluid clearance by instilling 1 ml of lactated Ringer's with 5 or50ng/ml of rHGF (Gcnentech) and 1:>I albumin into the distal airspaces of anesthetized ventilated rats. The increase in the alveolar concentration of the instilled '-4I-albumin over 1 hour was used to measure clearance of liquid fron] the alveoli. Data as mean ±SD. 'P<:0.0.i._____________________________________ Final/Instilled Alveolar liquid Experimental Studies No. 125l-albumin Clearance Control 5 1.25 ,t O.Ob 20.0 ±4.0 rHGF 5 ot 5(1 ng/ml 7 1.47 ± 0.16 31.4 ± 7,0 In additon to its recently reported motogenic and mitogenic effects on alveolar epithelial cells, these results provide evidence that HGF can augment alveolar fluid clearance. (Supported in part by NIH HL51K54).

Original languageEnglish (US)
Pages (from-to)A354
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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