Hepatocellular carcinoma model cell lines with two distinct migration modes

In Jeong Lee, Zhong Shu Li, Young Nam Lee, Xue Jun Jin, Jeong Hyung Lee, Seon Young Kim, Nam Soon Kim, Dong Chul Lee, Heun Sik Lee, Suk Jin Yang, Soo Jung Kim, Young Il Yeom

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Migration is an essential feature of metastatic cancer cells. To understand how motility is regulated in hepatocellular carcinoma, we analyzed gene expression profiles of mouse model cell lines we established from transgenic mice carrying SV40 large T antigen. A non-motile HC9 cell line was isolated from mouse liver tumors, and two additional cell lines, HCM1 and HCM4, were derived from HC9 cells. We found that both HCM1 and HCM4 cells were substantially more migratory than HC9, and that HCM1 generated tumor nodules in nude mice. In contrast to HCM4 cells that exhibited mesenchymal cell-type gene expression similar to HC9 cells, HCM1 cells appeared to have undergone a mesenchymal-amoeboidal transition. Thus, HCM1 and HCM4 cells have distinct migration and gene expression patterns, and together with HC9 cells, they can serve as model cell lines for understanding how migration is acquired and controlled in hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)1217-1227
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number4
DOIs
StatePublished - Aug 11 2006

Keywords

  • Gene expression profiles
  • Hepatocellular carcinoma
  • Mesenchymal
  • Mesenchymal-amoeboidal transition
  • Migration

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Lee, I. J., Li, Z. S., Lee, Y. N., Jin, X. J., Lee, J. H., Kim, S. Y., Kim, N. S., Lee, D. C., Lee, H. S., Yang, S. J., Kim, S. J., & Yeom, Y. I. (2006). Hepatocellular carcinoma model cell lines with two distinct migration modes. Biochemical and Biophysical Research Communications, 346(4), 1217-1227. https://doi.org/10.1016/j.bbrc.2006.06.030