Hepatic tyrosine-phosphorylated proteins identified and localized following in vivo inhibition of protein tyrosine phosphatases

effects of H2O2 and vanadate administration into rat livers

Yaron R. Hadari, Benjamin Geiger, Orna Nadiv, Ilana Sabanay, Charles Roberts, Derek LeRoith, Yehiel Zick

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Injection of a combination of H2O2 and vanadate (H/V) into the portal vein of rat livers resulted in inhibition of protein tyrosine phosphatase activity and led to a dramatic enhanced in vivo protein tyrosine phosphorylation. Some of the phosphorylated proteins were identified as the β-subunit of the insulin receptor, the insulin receptor substrate 1 (ppl85), PLC-γ (pp145), and a 100 kDa PLC-γ-associated protein. Immunofluorescense and immune electron microscopy of frozen liver sections with anti-P-Tyr antibodies revealed that most of the tyrosine-phosphorylated proteins are localized in close proximity to the plasma membrane in intercellular adherence junctions and tight junction regions. This close in vivo association between membranal protein tyrosine kinases, their target proteins, and cytoskeletal elements could enable formation of 'signaling complexes' which may play a role in transmembrane signal transduction. By affinity chromatography over immobilized anti-P-Tyr antibodies, a large number of these tyrosine-phosphorylated proteins were partially purified.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume97
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Protein Tyrosine Phosphatases
Vanadates
Liver
Tyrosine
Rats
Programmable logic controllers
Proteins
Affinity chromatography
Insulin Receptor Substrate Proteins
Signal transduction
Phosphorylation
Cytoskeletal Proteins
Intercellular Junctions
Antibodies
Tight Junctions
Insulin Receptor
Frozen Sections
Cell membranes
Portal Vein
Affinity Chromatography

Keywords

  • HO-vanadate
  • Protein tyrosine kinase
  • Tyrosine phosphorylation (Rat liver)

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hepatic tyrosine-phosphorylated proteins identified and localized following in vivo inhibition of protein tyrosine phosphatases : effects of H2O2 and vanadate administration into rat livers. / Hadari, Yaron R.; Geiger, Benjamin; Nadiv, Orna; Sabanay, Ilana; Roberts, Charles; LeRoith, Derek; Zick, Yehiel.

In: Molecular and Cellular Endocrinology, Vol. 97, No. 1-2, 1993, p. 9-17.

Research output: Contribution to journalArticle

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