Hepatic α-smooth muscle actin expression in hepatitis C patients before and after interferon therapy

Akram Khan, J. E. Poulos, E. M. Brunt, L. Li, H. Solomon, R. S. Britton, B. R. Bacon, A. M. Di Bisceglie

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background/Aims: The mechanism of hepatic fibrogenesis with chronic viral hepatitis is not well understood. Persistent activation of hepatic stellate cells is felt to play a role in the development of fibrogenesis and progression to cirrhosis. Methodology: We determined the expression of hepatic α-smooth muscle actin, a marker of hepatic stellate cell activation, in 29 patients with chronic hepatitis C and varying degrees of liver injury and fibrosis. In addition to a baseline evaluation, we assessed the effect of interferon therapy on α-smooth muscle actin expression in 11 patients, including 6 with a sustained response to therapy. Specimens were evaluated by light microscopy for grade of inflammation and stage of fibrosis. Expression of α-smooth muscle actin was assessed semiquantitatively by immunohistochemical staining. Results: At baseline, all patients had α-smooth muscle actin expressed within the liver without an obvious correlation with the severity of liver injury. However, among sustained responders, a reduction in hepatic necroinflammatory activity was associated with a trend towards a decrease in α-smooth muscle actin expression. This however did not reach statistical significance. Conclusions: Hepatic α-smooth muscle actin expression, as a marker of hepatic stellate cell activation appears reversible and tends to correlate with necroinflammatory activity.

Original languageEnglish (US)
Pages (from-to)212-215
Number of pages4
JournalHepato-Gastroenterology
Volume48
Issue number37
StatePublished - 2001
Externally publishedYes

Fingerprint

Hepatitis C
Interferons
Smooth Muscle
Actins
Hepatic Stellate Cells
Liver
Fibrosis
Therapeutics
Wounds and Injuries
Chronic Hepatitis C
Chronic Hepatitis
Liver Cirrhosis
Microscopy
Staining and Labeling
Inflammation
Light

Keywords

  • Alpha smooth muscle actin
  • Fibrogenesis
  • Hepatitis C
  • Interferon
  • Stellate cell

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Khan, A., Poulos, J. E., Brunt, E. M., Li, L., Solomon, H., Britton, R. S., ... Di Bisceglie, A. M. (2001). Hepatic α-smooth muscle actin expression in hepatitis C patients before and after interferon therapy. Hepato-Gastroenterology, 48(37), 212-215.

Hepatic α-smooth muscle actin expression in hepatitis C patients before and after interferon therapy. / Khan, Akram; Poulos, J. E.; Brunt, E. M.; Li, L.; Solomon, H.; Britton, R. S.; Bacon, B. R.; Di Bisceglie, A. M.

In: Hepato-Gastroenterology, Vol. 48, No. 37, 2001, p. 212-215.

Research output: Contribution to journalArticle

Khan, A, Poulos, JE, Brunt, EM, Li, L, Solomon, H, Britton, RS, Bacon, BR & Di Bisceglie, AM 2001, 'Hepatic α-smooth muscle actin expression in hepatitis C patients before and after interferon therapy', Hepato-Gastroenterology, vol. 48, no. 37, pp. 212-215.
Khan, Akram ; Poulos, J. E. ; Brunt, E. M. ; Li, L. ; Solomon, H. ; Britton, R. S. ; Bacon, B. R. ; Di Bisceglie, A. M. / Hepatic α-smooth muscle actin expression in hepatitis C patients before and after interferon therapy. In: Hepato-Gastroenterology. 2001 ; Vol. 48, No. 37. pp. 212-215.
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