Hemozoin produced by mammals confers heme tolerance

Rini H. Pek, Xiaojing Yuan, Nicole Rietzschel, Jianbing Zhang, Laurie Jackson, Eiji Nishibori, Ana Ribeiro, William Simmons, Jaya Jagadeesh, Hiroshi Sugimoto, Md Zahidul Alam, Lisa Garrett, Malay Haldar, Martina Ralle, John D. Phillips, David M. Bodine, Iqbal Hamza

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Free heme is cytotoxic as exemplified by hemolytic diseases and genetic deficiencies in heme recycling and detoxifying pathways. Thus, intracellular accumulation of heme has not been observed in mammalian cells to date. Here we show that mice deficient for the heme transporter SLC48A1 (also known as HRG1) accumulate over ten-fold excess heme in reticuloendothelial macrophage lysosomes that are 10 to 100 times larger than normal. Macrophages tolerate these high concentrations of heme by crystallizing them into hemozoin, which heretofore has only been found in blood-feeding organisms. SLC48A1 deficiency results in impaired erythroid maturation and an inability to systemically respond to iron deficiency. Complete heme tolerance requires a fully-operational heme degradation pathway as haplo insufficiency of HMOX1 combined with SLC48A1 inactivation causes perinatal lethality demonstrating synthetic lethal interactions between heme transport and degradation. Our studies establish the formation of hemozoin by mammals as a previously unsuspected heme tolerance pathway.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Oct 1 2019

Fingerprint

Mammals
Heme
Macrophages
hemozoin
Deficiency Diseases
Degradation
Inborn Genetic Diseases
Recycling
Lysosomes
Blood
Iron
Cells

Keywords

  • anemia
  • biochemistry
  • cell biology
  • chemical biology
  • heme
  • hemozoin
  • iron
  • lysosome
  • macrophages
  • mouse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Pek, R. H., Yuan, X., Rietzschel, N., Zhang, J., Jackson, L., Nishibori, E., ... Hamza, I. (2019). Hemozoin produced by mammals confers heme tolerance. eLife, 8. https://doi.org/10.7554/eLife.49503

Hemozoin produced by mammals confers heme tolerance. / Pek, Rini H.; Yuan, Xiaojing; Rietzschel, Nicole; Zhang, Jianbing; Jackson, Laurie; Nishibori, Eiji; Ribeiro, Ana; Simmons, William; Jagadeesh, Jaya; Sugimoto, Hiroshi; Alam, Md Zahidul; Garrett, Lisa; Haldar, Malay; Ralle, Martina; Phillips, John D.; Bodine, David M.; Hamza, Iqbal.

In: eLife, Vol. 8, 01.10.2019.

Research output: Contribution to journalArticle

Pek, RH, Yuan, X, Rietzschel, N, Zhang, J, Jackson, L, Nishibori, E, Ribeiro, A, Simmons, W, Jagadeesh, J, Sugimoto, H, Alam, MZ, Garrett, L, Haldar, M, Ralle, M, Phillips, JD, Bodine, DM & Hamza, I 2019, 'Hemozoin produced by mammals confers heme tolerance', eLife, vol. 8. https://doi.org/10.7554/eLife.49503
Pek RH, Yuan X, Rietzschel N, Zhang J, Jackson L, Nishibori E et al. Hemozoin produced by mammals confers heme tolerance. eLife. 2019 Oct 1;8. https://doi.org/10.7554/eLife.49503
Pek, Rini H. ; Yuan, Xiaojing ; Rietzschel, Nicole ; Zhang, Jianbing ; Jackson, Laurie ; Nishibori, Eiji ; Ribeiro, Ana ; Simmons, William ; Jagadeesh, Jaya ; Sugimoto, Hiroshi ; Alam, Md Zahidul ; Garrett, Lisa ; Haldar, Malay ; Ralle, Martina ; Phillips, John D. ; Bodine, David M. ; Hamza, Iqbal. / Hemozoin produced by mammals confers heme tolerance. In: eLife. 2019 ; Vol. 8.
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