Hemodynamic forces regulate collagen deposition in the embryonic chicken outflow tract

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Alterations in blood flow at early embryonic stages can lead to detrimental remodeling and heart defects, but these structural adaptations are not well understood. We hypothesize that deposition of collagens will be increased as shear stress is increased - leading to a stiffer wall. To test this hypothesis a suture (OTB) was tightened around the outflow tract (OFT) of stage HH18 chick embryos for 24 hours to reduce cross sectional area of the lumen. Sham and OTB embryos were immunostained for collagen I, III, VI and XIV, imaged with confocal microscopy, and staining was quantified by grayscale analysis. Changes in fibril collagens I and III were not observed, however deposition of collagens VI and XIV increased in a degree-of-constriction dependent manner. The observed increase in collagen VI and XIV deposition suggests they play a key role in structural adaptation to increased hemodynamic pressure.

Original languageEnglish (US)
Title of host publicationASME 2013 Summer Bioengineering Conference, SBC 2013
DOIs
StatePublished - Dec 1 2013
EventASME 2013 Summer Bioengineering Conference, SBC 2013 - Sunriver, OR, United States
Duration: Jun 26 2013Jun 29 2013

Publication series

NameASME 2013 Summer Bioengineering Conference, SBC 2013
Volume1 A

Other

OtherASME 2013 Summer Bioengineering Conference, SBC 2013
CountryUnited States
CitySunriver, OR
Period6/26/136/29/13

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ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Mechanical Engineering

Cite this

Rennie, M. Y., Danilchik, M., Thornburg, K. L., & Rugonyi, S. (2013). Hemodynamic forces regulate collagen deposition in the embryonic chicken outflow tract. In ASME 2013 Summer Bioengineering Conference, SBC 2013 (ASME 2013 Summer Bioengineering Conference, SBC 2013; Vol. 1 A). https://doi.org/10.1115/SBC2013-14299