Heme oxygenase expression in cultured human trophoblast cells during in vitro differentiation: Effects of hypoxia

D. Newby, F. Cousins, Leslie Myatt, F. Lyall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Heme oxygenases (HO-1 and HO-2) are responsible for the production of carbon monoxide, a vasodilator. HO is important in controlling placental blood flow and expression can be sensitive to oxygen. We previously reported a reduction in HO-2 expression in placentae obtained from patients with pre-eclampsia or living at high altitude, both associated with placental hypoxia. Thus we hypothesized that HO expression in cultured trophoblasts would be altered by exposure to hypoxia. HO-1 and HO-2 expression was assessed in trophoblast cell cultures following exposure to different oxygen environments. Western blot analyses showed that HO-1 expression in syncytiotrophoblast was significantly lower than in cytotrophoblasts in standard conditions (p2 for various times. However, exposure of syncytiotrophoblast cultures to hypoxia for 12 h resulted in a significant reduction in HO-1 expression (p

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalPlacenta
Volume26
Issue number2-3
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Heme Oxygenase (Decyclizing)
Trophoblasts
Oxygen
Heme Oxygenase-1
Carbon Monoxide
Pre-Eclampsia
Vasodilator Agents
Placenta
Cell Culture Techniques
Western Blotting
Hypoxia
In Vitro Techniques

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Heme oxygenase expression in cultured human trophoblast cells during in vitro differentiation : Effects of hypoxia. / Newby, D.; Cousins, F.; Myatt, Leslie; Lyall, F.

In: Placenta, Vol. 26, No. 2-3, 02.2005, p. 201-209.

Research output: Contribution to journalArticle

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