TY - JOUR
T1 - Hematocrit dependence of flow signal in optical coherence tomography angiography
AU - Yang, Jianlong
AU - Su, Johnny
AU - Wang, Jie
AU - Men, Silu
AU - Jia, Yali
AU - Huang, David
AU - Liu, Gangjun
N1 - Funding Information:
This research was funded by Oregon Health & Science Foundation, National Institutes of Health Grants DP3 DK104397, R01 EY024544, R01 EY023285 and R01 EY018184, unrestricted departmental funding from Research to Prevent Blindness (New York, NY), and P30 EY010572 from the National Institutes of Health (Bethesda, MD).
Publisher Copyright:
© 2017 Optical Society of America.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The hematocrit dependence of flow signal (split-spectrum amplitude decorrelation angiography-SSADA decorrelation value) was investigated in this paper. Based on the normalized field temporal correlation function and concentration dependent particle scattering properties, the relationship between hematocrit and flow signal was analytically derived. Experimental verification of the relationship was performed with custom-designed microfluidic chips and human blood with 45%, 40% and 32% hematocrit. It was found that, in large flow channels and blood vessels, the normal hematocrit is near the decorrelation saturation point and therefore a change in hematocrit has little effect on the SSADA decorrelation value (flow signal). However, in narrow channels in the capillary size range, the effective hematocrit (adjusted for the overlap between OCT beam and channel) is in the range of 6.7-9.5% and therefore variation in hematocrit does significantly affect the flow signal.
AB - The hematocrit dependence of flow signal (split-spectrum amplitude decorrelation angiography-SSADA decorrelation value) was investigated in this paper. Based on the normalized field temporal correlation function and concentration dependent particle scattering properties, the relationship between hematocrit and flow signal was analytically derived. Experimental verification of the relationship was performed with custom-designed microfluidic chips and human blood with 45%, 40% and 32% hematocrit. It was found that, in large flow channels and blood vessels, the normal hematocrit is near the decorrelation saturation point and therefore a change in hematocrit has little effect on the SSADA decorrelation value (flow signal). However, in narrow channels in the capillary size range, the effective hematocrit (adjusted for the overlap between OCT beam and channel) is in the range of 6.7-9.5% and therefore variation in hematocrit does significantly affect the flow signal.
KW - Medical and biological imaging
KW - Ophthalmic optics and devices
KW - Optical coherence tomography
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U2 - 10.1364/BOE.8.000776
DO - 10.1364/BOE.8.000776
M3 - Article
AN - SCOPUS:85011579661
VL - 8
SP - 776
EP - 789
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 2
M1 - #276240
ER -