HCMV gB shares structural and functional properties with gB proteins from other herpesviruses

Sapna Sharma, Todd W. Wisner, David Johnson, Ekaterina E. Heldwein

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Glycoprotein B (gB) facilitates HCMV entry into cells by binding receptors and mediating membrane fusion. The crystal structures of gB ectodomains from HSV-1 and EBV are available, but little is known about the HCMV gB structure. Using multiangle light scattering and electron microscopy, we show here that HCMV gB ectodomain is a trimer with the overall shape similar to HSV-1 and EBV gB ectodomains. HCMV gB ectodomain forms rosettes similar to rosettes formed by EBV gB and the postfusion forms of other viral fusogens. Substitution of several bulky hydrophobic residues within the putative fusion loops with more hydrophilic residues reduced rosette formation and abolished cell fusion. We propose that like gB proteins from HSV-1 and EBV, HCMV gB has two internal hydrophobic fusion loops that likely interact with target membranes. Our work establishes structural and functional similarities between gB proteins from three subfamilies of herpesviruses.

Original languageEnglish (US)
Pages (from-to)239-249
Number of pages11
JournalVirology
Volume435
Issue number2
DOIs
StatePublished - Jan 20 2013

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Herpesviridae
Glycoproteins
Human Herpesvirus 4
Rosette Formation
Membrane Fusion
Cell Fusion
IgA receptor
Electron Microscopy
Light
Membranes
Simplexvirus glycoprotein B

Keywords

  • Cytomegalovirus
  • Entry
  • Fusion loop
  • Glycoprotein B
  • Glycosylation
  • Herpesvirus
  • Membrane fusion
  • Rosette

ASJC Scopus subject areas

  • Virology

Cite this

HCMV gB shares structural and functional properties with gB proteins from other herpesviruses. / Sharma, Sapna; Wisner, Todd W.; Johnson, David; Heldwein, Ekaterina E.

In: Virology, Vol. 435, No. 2, 20.01.2013, p. 239-249.

Research output: Contribution to journalArticle

Sharma, Sapna ; Wisner, Todd W. ; Johnson, David ; Heldwein, Ekaterina E. / HCMV gB shares structural and functional properties with gB proteins from other herpesviruses. In: Virology. 2013 ; Vol. 435, No. 2. pp. 239-249.
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