Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing

Sasan Amini, Dmitry Pushkarev, Lena Christiansen, Emrah Kostem, Tom Royce, Casey Turk, Natasha Pignatelli, Andrew Adey, Jacob O. Kitzman, Kandaswamy Vijayan, Mostafa Ronaghi, Jay Shendure, Kevin L. Gunderson, Frank J. Steemers

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Haplotype-resolved genome sequencing enables the accurate interpretation of medically relevant genetic variation, deep inferences regarding population history and non-invasive prediction of fetal genomes. We describe an approach for genome-wide haplotyping based on contiguity-preserving transposition (CPT-seq) and combinatorial indexing. Tn5 transposition is used to modify DNA with adaptor and index sequences while preserving contiguity. After DNA dilution and compartmentalization, the transposase is removed, resolving the DNA into individually indexed libraries. The libraries in each compartment, enriched for neighboring genomic elements, are further indexed via PCR. Combinatorial 96-plex indexing at both the transposition and PCR stage enables the construction of phased synthetic reads from each of the nearly 10,000 'virtual compartments'. We demonstrate the feasibility of this method by assembling >95% of the heterozygous variants in a human genome into long, accurate haplotype blocks (N50 = 1.4-2.3 Mb). The rapid, scalable and cost-effective workflow could enable haplotype resolution to become routine in human genome sequencing.

Original languageEnglish (US)
Pages (from-to)1343-1349
Number of pages7
JournalNature Genetics
Volume46
Issue number12
DOIs
StatePublished - Dec 11 2014
Externally publishedYes

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Haplotypes
Genome
Human Genome
Libraries
DNA
Transposases
Polymerase Chain Reaction
Workflow
Costs and Cost Analysis
Population

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Amini, S., Pushkarev, D., Christiansen, L., Kostem, E., Royce, T., Turk, C., ... Steemers, F. J. (2014). Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing. Nature Genetics, 46(12), 1343-1349. https://doi.org/10.1038/ng.3119

Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing. / Amini, Sasan; Pushkarev, Dmitry; Christiansen, Lena; Kostem, Emrah; Royce, Tom; Turk, Casey; Pignatelli, Natasha; Adey, Andrew; Kitzman, Jacob O.; Vijayan, Kandaswamy; Ronaghi, Mostafa; Shendure, Jay; Gunderson, Kevin L.; Steemers, Frank J.

In: Nature Genetics, Vol. 46, No. 12, 11.12.2014, p. 1343-1349.

Research output: Contribution to journalArticle

Amini, S, Pushkarev, D, Christiansen, L, Kostem, E, Royce, T, Turk, C, Pignatelli, N, Adey, A, Kitzman, JO, Vijayan, K, Ronaghi, M, Shendure, J, Gunderson, KL & Steemers, FJ 2014, 'Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing', Nature Genetics, vol. 46, no. 12, pp. 1343-1349. https://doi.org/10.1038/ng.3119
Amini S, Pushkarev D, Christiansen L, Kostem E, Royce T, Turk C et al. Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing. Nature Genetics. 2014 Dec 11;46(12):1343-1349. https://doi.org/10.1038/ng.3119
Amini, Sasan ; Pushkarev, Dmitry ; Christiansen, Lena ; Kostem, Emrah ; Royce, Tom ; Turk, Casey ; Pignatelli, Natasha ; Adey, Andrew ; Kitzman, Jacob O. ; Vijayan, Kandaswamy ; Ronaghi, Mostafa ; Shendure, Jay ; Gunderson, Kevin L. ; Steemers, Frank J. / Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing. In: Nature Genetics. 2014 ; Vol. 46, No. 12. pp. 1343-1349.
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