Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation

Moritoshi Hirono, Charlotte S. Denis, Guy P. Richardson, Peter Barr-Gillespie

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

After opening in response to mechanical stimuli, hair cell transduction channels adapt with fast and slow mechanisms that each depend on Ca 2+. We demonstrate here that transduction and adaptation require phosphatidylinositol 4,5-bisphosphate (PIP2) for normal kinetics. PIP2 has a striking distribution in hair cells, being excluded from the basal region of hair bundles and apical surfaces of frog saccular hair cells. Localization of a phosphatidylinositol lipid phosphatase, Ptprq, to these PIP2-free domains suggests that Ptprq maintains low PIP2 levels there. Depletion of PIP2 by inhibition of phosphatidylinositol 4-kinase or sequestration by aminoglycosides reduces the rates of fast and slow adaptation. PIP2 and other anionic phospholipids bind directly to the IQ domains of myosin-1c, the motor that mediates slow adaptation, permitting a strong interaction with membranes and likely regulating the motor's activity. PIP2 depletion also causes a loss in transduction current. PIP 2 therefore plays an essential role in hair cell adaptation and transduction.

Original languageEnglish (US)
Pages (from-to)309-320
Number of pages12
JournalNeuron
Volume44
Issue number2
DOIs
StatePublished - Oct 14 2004

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Phosphatidylinositols
1-Phosphatidylinositol 4-Kinase
Aminoglycosides
Myosins
Phosphoric Monoester Hydrolases
Anura
Phospholipids
Motor Activity
Lipids
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)

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Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation. / Hirono, Moritoshi; Denis, Charlotte S.; Richardson, Guy P.; Barr-Gillespie, Peter.

In: Neuron, Vol. 44, No. 2, 14.10.2004, p. 309-320.

Research output: Contribution to journalArticle

Hirono, Moritoshi ; Denis, Charlotte S. ; Richardson, Guy P. ; Barr-Gillespie, Peter. / Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation. In: Neuron. 2004 ; Vol. 44, No. 2. pp. 309-320.
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