hADA3 is required for p53 activity

Ting Wang, Takahiko Kobayashi, Rishu Takimoto, Alex Denes, Eric L. Snyder, Wafik S. El-Deiry, Rainer K. Brachmann

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.

Original languageEnglish (US)
Pages (from-to)6404-6413
Number of pages10
JournalEMBO Journal
Volume20
Issue number22
DOIs
StatePublished - Nov 15 2001
Externally publishedYes

Fingerprint

DNA Damage
Cells
Apoptosis
Tumor Suppressor Protein p53
Histone Acetyltransferases
Phosphorylation
DNA
Post Translational Protein Processing
Cell Cycle Checkpoints
Yeast
Assays
Transcription Factors
Yeasts
Neoplasms
Proteins

Keywords

  • Apoptosis
  • DNA damage
  • hADA3
  • Histone acetyltransferase
  • p53

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Wang, T., Kobayashi, T., Takimoto, R., Denes, A., Snyder, E. L., El-Deiry, W. S., & Brachmann, R. K. (2001). hADA3 is required for p53 activity. EMBO Journal, 20(22), 6404-6413. https://doi.org/10.1093/emboj/20.22.6404

hADA3 is required for p53 activity. / Wang, Ting; Kobayashi, Takahiko; Takimoto, Rishu; Denes, Alex; Snyder, Eric L.; El-Deiry, Wafik S.; Brachmann, Rainer K.

In: EMBO Journal, Vol. 20, No. 22, 15.11.2001, p. 6404-6413.

Research output: Contribution to journalArticle

Wang, T, Kobayashi, T, Takimoto, R, Denes, A, Snyder, EL, El-Deiry, WS & Brachmann, RK 2001, 'hADA3 is required for p53 activity', EMBO Journal, vol. 20, no. 22, pp. 6404-6413. https://doi.org/10.1093/emboj/20.22.6404
Wang T, Kobayashi T, Takimoto R, Denes A, Snyder EL, El-Deiry WS et al. hADA3 is required for p53 activity. EMBO Journal. 2001 Nov 15;20(22):6404-6413. https://doi.org/10.1093/emboj/20.22.6404
Wang, Ting ; Kobayashi, Takahiko ; Takimoto, Rishu ; Denes, Alex ; Snyder, Eric L. ; El-Deiry, Wafik S. ; Brachmann, Rainer K. / hADA3 is required for p53 activity. In: EMBO Journal. 2001 ; Vol. 20, No. 22. pp. 6404-6413.
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