Guanine nucleotides induce tyrosine phosphorylation and activation of the respiratory burst in neutrophils

P. E. Nasmith, Gordon Mills, S. Grinstein

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Activation of the NADPH oxidase was examined in electrically permeabilized human neutrophils exposed to non-hydrolysable guanine nucleotides. Guanosine 5'-[γ-thio]triphosphate (GTP[S]) induced a marked increase in the rate of O2 consumption, which was partially resistant to staurosporine, an inhibhitor of protein kinase C, under conditions where the response to diacylglycerol was virtually abolished. The respiratory burst elicited by GTP[S] was dependent on the presence of ATP and Mg2+, suggesting involvement of phosphorylation reactions. Accordingly, phosphoprotein formation was greatly stimulated by the guanine nucleotide. The polypeptide phosphorylation pattern induced by GTP[S] was similar to, but not identical with, that observed with diacylglycerol, indicating the activation of kinases other than protein kinase C by the guanine nucleotide. The possible involvement of tyrosine kinases was assessed by immunoblotting using anti-phosphotyrosine antibodies. Treatment of electroporated cells with GTP[S] stimulated the accumulation of tyrosine-phosphorylated proteins. This effect was not induced by diacylglycerol, indicating the tyrosine phosphorylation is not secondary to stimulation of protein kinase C. The results indicate that, in neutrophils, activated G-proteins can stimulate tyrosine kinase and/or inhibit tyrosine phosphatase activity. Changes in the amounts of tyrosine-phosphorylated proteins may signal activation of the respiratory burst.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalBiochemical Journal
Volume257
Issue number3
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Phosphorylation
Guanine Nucleotides
Respiratory Burst
Guanosine Triphosphate
Tyrosine
Diglycerides
Neutrophils
Chemical activation
Protein Kinase C
Protein-Tyrosine Kinases
Phosphotyrosine
Staurosporine
Guanosine
NADPH Oxidase
Phosphoproteins
GTP-Binding Proteins
Phosphoric Monoester Hydrolases
Immunoblotting
Anti-Idiotypic Antibodies
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Guanine nucleotides induce tyrosine phosphorylation and activation of the respiratory burst in neutrophils. / Nasmith, P. E.; Mills, Gordon; Grinstein, S.

In: Biochemical Journal, Vol. 257, No. 3, 01.01.1989, p. 893-897.

Research output: Contribution to journalArticle

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