Guanine nucleotides and pertussis toxin alter agonist binding to rat septal V1-vasopressin receptors

Michael W. Swank; Daniel M. Dorsa

doi:10.1016/1044-7431(90)90014-U

Guanine nucleotides and pertussis toxin alter agonist binding to rat septal V₁-vasopressin receptors

Michael W. Swank, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To investigate whether the rat brain vasopressin receptor is coupled to phospholipase C by a GTP-binding protein, the effects of guanine nucleotides and pertussis toxin on agonist binding to septal membranes were examined. Synaptic plasma membranes were prepared from rat septal tissue and binding of [³H]AVP was examined in the presence of guanine nucleotides at a concentration of 1 mM. Saturation isotherms revealed that all guanine nucleotides significantly increased the dissociation constant K_d, and the nonhydrolyzable GTP analogs GTPγS and Gpp(NH)p had the most pronounced effect. Treatment of membranes with pertussis toxin reduced the specific binding of 2 nM [³H]AVP by over 50%. These data suggest that the septal vasopressin receptor is coupled to phospholipase C via a G_i- or G_o-like GTP-binding protein.

Original language	English (US)
Pages (from-to)	117-120
Number of pages	4
Journal	Molecular and Cellular Neuroscience
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/1044-7431(90)90014-U
State	Published - Oct 1990
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience
Cell Biology

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10.1016/1044-7431(90)90014-U

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title = "Guanine nucleotides and pertussis toxin alter agonist binding to rat septal V1-vasopressin receptors",

abstract = "To investigate whether the rat brain vasopressin receptor is coupled to phospholipase C by a GTP-binding protein, the effects of guanine nucleotides and pertussis toxin on agonist binding to septal membranes were examined. Synaptic plasma membranes were prepared from rat septal tissue and binding of [3H]AVP was examined in the presence of guanine nucleotides at a concentration of 1 mM. Saturation isotherms revealed that all guanine nucleotides significantly increased the dissociation constant Kd, and the nonhydrolyzable GTP analogs GTPγS and Gpp(NH)p had the most pronounced effect. Treatment of membranes with pertussis toxin reduced the specific binding of 2 nM [3H]AVP by over 50%. These data suggest that the septal vasopressin receptor is coupled to phospholipase C via a Gi- or Go-like GTP-binding protein.",

author = "Swank, {Michael W.} and Dorsa, {Daniel M.}",

note = "Funding Information: These studies were supported hy NIH Grant NS20311, (Grant for Neurobiology T32GMO7108, and the research I)epartment of Veterans Affairs.",

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T1 - Guanine nucleotides and pertussis toxin alter agonist binding to rat septal V1-vasopressin receptors

AU - Swank, Michael W.

AU - Dorsa, Daniel M.

N1 - Funding Information: These studies were supported hy NIH Grant NS20311, (Grant for Neurobiology T32GMO7108, and the research I)epartment of Veterans Affairs.

PY - 1990/10

Y1 - 1990/10

N2 - To investigate whether the rat brain vasopressin receptor is coupled to phospholipase C by a GTP-binding protein, the effects of guanine nucleotides and pertussis toxin on agonist binding to septal membranes were examined. Synaptic plasma membranes were prepared from rat septal tissue and binding of [3H]AVP was examined in the presence of guanine nucleotides at a concentration of 1 mM. Saturation isotherms revealed that all guanine nucleotides significantly increased the dissociation constant Kd, and the nonhydrolyzable GTP analogs GTPγS and Gpp(NH)p had the most pronounced effect. Treatment of membranes with pertussis toxin reduced the specific binding of 2 nM [3H]AVP by over 50%. These data suggest that the septal vasopressin receptor is coupled to phospholipase C via a Gi- or Go-like GTP-binding protein.

AB - To investigate whether the rat brain vasopressin receptor is coupled to phospholipase C by a GTP-binding protein, the effects of guanine nucleotides and pertussis toxin on agonist binding to septal membranes were examined. Synaptic plasma membranes were prepared from rat septal tissue and binding of [3H]AVP was examined in the presence of guanine nucleotides at a concentration of 1 mM. Saturation isotherms revealed that all guanine nucleotides significantly increased the dissociation constant Kd, and the nonhydrolyzable GTP analogs GTPγS and Gpp(NH)p had the most pronounced effect. Treatment of membranes with pertussis toxin reduced the specific binding of 2 nM [3H]AVP by over 50%. These data suggest that the septal vasopressin receptor is coupled to phospholipase C via a Gi- or Go-like GTP-binding protein.

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Guanine nucleotides and pertussis toxin alter agonist binding to rat septal V₁-vasopressin receptors

Abstract

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