Growth hormone stimulates insulin gene expression in cultured human fetal pancreatic islets

B. Formby, A. Ullrich, Lisa Coussens, L. Walker, C. M. Peterson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The total body mass of the human fetus increases about 100-fold from 10-20 weeks of gestation, and peak serum GH concentrations occur at 20 weeks. Since insulin has an essential growth-promoting influence in the fetus, these experiments were designed to determine whether GH can function as a growth factor with insulin-releasing activity by stimulating insulin gene expression during embryogenesis. Pancreatic islets were isolated from human fetuses (n = 36) of 18-22 weeks gestational age. Insulin gene expression was quantified by measuring insulin mRNA by blot hybridization analysis using a [32P] cDNA probe encoding human insulin. We found that by 48 h of culture insulin gene expression was stimulated by 1.25 μg recombinant human GH/mL medium to 216% of the control value (n = 2). Insulin secretory capacity was expressed as a fractional stimulation ratio (FSR = F2/F1) of insulin release rates during two successive 1-h static incubations. After 48 h of culture 1.25 μg/mL GH stimulated the FSR value to 273% of the control value (n = 5). We conclude that recombinant human GH significantly enhances the steady state level of insulin mRNA concurrent with an increase in insulin secretory capacity, hence providing evidence for a regulatory function of GH on insulin gene expression during fetal development.

Original languageEnglish (US)
Pages (from-to)1075-1079
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume66
Issue number5
StatePublished - 1988
Externally publishedYes

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Islets of Langerhans
Gene expression
Growth Hormone
Insulin
Gene Expression
Fetus
Messenger RNA
Fetal Development
Human Body
Gestational Age
Embryonic Development
Intercellular Signaling Peptides and Proteins
Complementary DNA
Pregnancy

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Growth hormone stimulates insulin gene expression in cultured human fetal pancreatic islets. / Formby, B.; Ullrich, A.; Coussens, Lisa; Walker, L.; Peterson, C. M.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 66, No. 5, 1988, p. 1075-1079.

Research output: Contribution to journalArticle

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