Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-I

Mitchell E. Geffner, Noelle Bersch, Barbara M. Lippe, Ronald (Ron) Rosenfeld, Raymond L. Hintz, David W. Golde

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

It has become evident that locally produced insulin-like growth factors-I and -II (IGF-I and IGF-II) play an important role in the mediation of GH action upon tissues. To explore this concept with respect to immunocompetent cells, we analyzed IGF production and clonogenic responsiveness of immortalized human T-cell lines established from seven normal controls and four Laron dwarfs. While the normal T-cell lines showed significant augmentation of basal colony formation in response to both IGF-I and GH, little increase in clonogenesis in response to GH was seen with the Laron T-cell lines. Assay of basal and GH-stimulated conditioned media demonstrated low, but measurable, levels of IGF-I and IGF-II from both normal and Laron T-cells. Under serum-free incubation conditions, GH stimulation of normal T-cell lines failed to generate significant increases in mean IGF-I or IGF-II concentrations. Only a marginal increase in the mean IGF-I concentration and no increase in the mean IGF-II concentration in conditioned medium were observed after GH stimulation of Laron T-cell lines. Nevertheless, the increased cloning efficiency of the normal T-cell lines in response to either GH or IGF-I was nearly completely abrogated by preincubation of cells with antibodies to either IGF-I or the type I IGF receptor. These studies, thus, support a role for locally generated IGF-I in the mediation of GH action on T-lymphocytes and indicate that this effect is mediated via the type I IGF receptor. (J Clin Endocrinol Metab 71: 464-469, 1990).

Original languageEnglish (US)
Pages (from-to)464-469
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume71
Issue number2
StatePublished - Aug 1990
Externally publishedYes

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T-cells
Insulin-Like Growth Factor I
Growth Hormone
Cells
Cell Line
T-Lymphocytes
Insulin-Like Growth Factor II
Growth
IGF Type 1 Receptor
Conditioned Culture Medium
Cloning
Organism Cloning
Assays
Tissue
Antibodies
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Geffner, M. E., Bersch, N., Lippe, B. M., Rosenfeld, R. R., Hintz, R. L., & Golde, D. W. (1990). Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-I. Journal of Clinical Endocrinology and Metabolism, 71(2), 464-469.

Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-I. / Geffner, Mitchell E.; Bersch, Noelle; Lippe, Barbara M.; Rosenfeld, Ronald (Ron); Hintz, Raymond L.; Golde, David W.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 71, No. 2, 08.1990, p. 464-469.

Research output: Contribution to journalArticle

Geffner, ME, Bersch, N, Lippe, BM, Rosenfeld, RR, Hintz, RL & Golde, DW 1990, 'Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-I', Journal of Clinical Endocrinology and Metabolism, vol. 71, no. 2, pp. 464-469.
Geffner, Mitchell E. ; Bersch, Noelle ; Lippe, Barbara M. ; Rosenfeld, Ronald (Ron) ; Hintz, Raymond L. ; Golde, David W. / Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-I. In: Journal of Clinical Endocrinology and Metabolism. 1990 ; Vol. 71, No. 2. pp. 464-469.
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