GROα in the fetomaternal and amniotic fluid compartments during pregnancy and parturition

Jose Cohen, Fabio Ghezzi, Roberto Romero, Alessandro Ghidini, Moshe Mazor, Jorge Tolosa, Luis F. Gonçalves, Ricardo Gomez

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

PROBLEM: GROα/MGSA is a new member of the chemokine superfamily CXC(α) and is produced by a variety of cells including macrophages, fibroblasts, epithelial, and endothelial cells, and keratinocytes. This chemokine has chemoattractant activity and may participate in neutrophil recruitment and activation during the course of intrauterine infection. This study was conducted to investigate the effect of labor and microbial invasion of the amniotic cavity (MIAC) on amniotic fluid, fetal, and maternal plasma GROα concentrations. METHOD: A cross-sectional study was designed using parameters that included gestational age, results of amniotic fluid (AF) cultures, and labor status at the time of amniocentesis. Fluid was retrieved by transabdominal amniocentesis. MIAC was defined as a positive amniotic fluid culture for bacteria. Umbilical cord blood was retrieved at the time of delivery. Amniotic fluid, maternal and fetal plasma GROα concentrations were measured with a sensitive and specific ELISA (Quantikine, R and D Systems, Minneapolis, MN). RESULTS: 1) GROα was detectable in amniotic fluid, umbilical cord, and maternal plasma samples; 2) GROα concentrations in amniotic fluid increased with advancing gestational age; 3) Both term and preterm gestations with MIAC were associated with higher amniotic fluid GROα concentrations than those with sterile amniotic fluid, independent of the labor status (term, MIAC, labor: median 2.7 ng/ml, range 1.4-12.7 vs. term, no MIAC, labor: median 2.1 ng/ml, range 0.7-3.4, vs term, no MIAC, no labor: median 1.9 ng/ml, range 1.8-4.2; P <0.005; preterm: MIAC median 5 ng/ml, range 0.6-47.9 vs. no MIAC: median 2.3 ng/ml, range 0.5-10; P <0.008); 4) A strong correlation was found between umbilical cord plasma GROα concentrations and neonatal neutrophil count, and between GROα concentrations and white blood cell count in the amniotic fluid (r = 0.67, P <0.0005 and r = 0.38, P <0.001, respectively). CONCLUSION: GROα is a physiologic constituent of amniotic fluid and cord blood. Amniotic fluid GROα concentrations increase with gestational age. Intrauterine infection both preterm and at term is associated with an increase in GROα concentrations of amniotic fluid, suggesting that GROα may play an important role in recruitment of neutrophils into the amniotic cavity.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume35
Issue number1
StatePublished - Jan 1996
Externally publishedYes

Fingerprint

Amniotic Fluid
Parturition
Pregnancy
Gestational Age
Amniocentesis
Neutrophil Infiltration
Umbilical Cord
Mothers
Fetal Blood
CXC Chemokines
Neutrophil Activation
Chemotactic Factors
Infection
Leukocyte Count
Keratinocytes
Chemokines
Neutrophils
Endothelial Cells
Fibroblasts
Cross-Sectional Studies

Keywords

  • Chemokine
  • Cytokines
  • GROα
  • Infection
  • Preterm labor

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Cohen, J., Ghezzi, F., Romero, R., Ghidini, A., Mazor, M., Tolosa, J., ... Gomez, R. (1996). GROα in the fetomaternal and amniotic fluid compartments during pregnancy and parturition. American Journal of Reproductive Immunology, 35(1), 23-29.

GROα in the fetomaternal and amniotic fluid compartments during pregnancy and parturition. / Cohen, Jose; Ghezzi, Fabio; Romero, Roberto; Ghidini, Alessandro; Mazor, Moshe; Tolosa, Jorge; Gonçalves, Luis F.; Gomez, Ricardo.

In: American Journal of Reproductive Immunology, Vol. 35, No. 1, 01.1996, p. 23-29.

Research output: Contribution to journalArticle

Cohen, J, Ghezzi, F, Romero, R, Ghidini, A, Mazor, M, Tolosa, J, Gonçalves, LF & Gomez, R 1996, 'GROα in the fetomaternal and amniotic fluid compartments during pregnancy and parturition', American Journal of Reproductive Immunology, vol. 35, no. 1, pp. 23-29.
Cohen, Jose ; Ghezzi, Fabio ; Romero, Roberto ; Ghidini, Alessandro ; Mazor, Moshe ; Tolosa, Jorge ; Gonçalves, Luis F. ; Gomez, Ricardo. / GROα in the fetomaternal and amniotic fluid compartments during pregnancy and parturition. In: American Journal of Reproductive Immunology. 1996 ; Vol. 35, No. 1. pp. 23-29.
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