Gravitational effects on the rearrangement of cytoplasmic components during axial formation in amphibian development

C. R. Phillips, B. Whalon, J. Moore, Michael Danilchik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The spatial positioning of the dorsal-ventral axis in the amphibian, Xenopus laevis, can be experimentally manipulated either by tipping the embryo relative to Earth's gravitational force vector or by centrifugation. Experimental evidence suggests that certain cytoplasmic components are redistributed during the first cell cycle and that these components are, in part, responsible for the establishment of this axis. Further studies indicate that at least some of the cytoplasmic components responsible for establishing this axis may be RNA. Recombinant cDNA and PCR technology are utilized to isolate DNA clones for messenger RNA which becomes spatially localized to the dorsal side of the embryo. These clones are being used to study the mechanisms of spatial localization and the function of the localized RNA transcripts.

Original languageEnglish (US)
Pages (from-to)225-235
Number of pages11
JournalAdvances in Space Research
Volume17
Issue number6-7
StatePublished - 1996

Fingerprint

Gravitational effects
gravitational effects
RNA
amphibian
embryos
Centrifugation
embryo
clone
ribonucleic acids
DNA
Earth (planet)
Cells
positioning
deoxyribonucleic acid
cycles
effect
Messenger RNA

ASJC Scopus subject areas

  • Space and Planetary Science
  • Astronomy and Astrophysics

Cite this

Gravitational effects on the rearrangement of cytoplasmic components during axial formation in amphibian development. / Phillips, C. R.; Whalon, B.; Moore, J.; Danilchik, Michael.

In: Advances in Space Research, Vol. 17, No. 6-7, 1996, p. 225-235.

Research output: Contribution to journalArticle

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