Grape seed extracts inhibit platelet aggregation by inhibiting protein tyrosine phosphatase

Joseph Wuxun Jin, Osamu Inoue, Katsue Suzuki-Inoue, Go Nishikawa, Yoshinori Kawakami, Masashi Hisamoto, Tohru Okuda, Yukio Ozaki

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Platelets play an important role in various thrombotic diseases, including myocardial infarction. Because red wine consumption is inversely associated with death due to ischemic heart diseases, the effects of grape components on platelet function have been extensively investigated. Grape seed extracts (GSEs) reportedly inhibit platelet aggregation; however, the underlying mechanism has not been elucidated. We discovered that GSEs inhibit platelet aggregation induced by collagen and thrombin-receptor agonist peptide and increase basal levels of tyrosine phosphorylation, which was also observed in the presence of a protein tyrosine phosphatase (PTP) inhibitor. An in vitro phosphatase assay indicated that GSE dose dependently inhibited PTP-1B and Src homology 2 domain-containing phosphatase-1 activity, which positively regulates platelet aggregation. We propose that GSEs inhibit platelet aggregation by inhibiting tyrosine phosphatase activity. Moreover, we showed that GSE ingestion inhibited platelet aggregation in mice without enhancing tail bleeding, implying that GSE supplementation might be beneficial to prevention of thrombotic diseases.

Original languageEnglish (US)
Pages (from-to)278-284
Number of pages7
JournalClinical and Applied Thrombosis/Hemostasis
Issue number3
StatePublished - Apr 2014
Externally publishedYes


  • collagen
  • grape seed extracts
  • platelets
  • polyphenols
  • protein tyrosine phosphatase
  • protein tyrosine phosphorylation

ASJC Scopus subject areas

  • Hematology


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