Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce rapid phosphorylation and activation of the proto-oncogene Raf-1 in a human factor-dependent myeloid cell line

Y. Kanakura, Brian Druker, K. W. Wood, H. J. Mamon, K. Okuda, T. M. Roberts, J. D. Griffin

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The product of the c-raf-1 proto-oncogene, Raf-1, is a 74,000-dalton cytoplasmic serine/threonine protein kinase that has been implicated as an intermediate in signal transduction mechanisms. In the human factor-dependent myeloid cell line MO7, both granulocyte-macrophage colony stimulating factor (GM-CSF) and Interleukin-3 (IL-3) were found to induce rapid, dose-dependent phosphorylation of Raf-1, which resulted in altered Raf-1 mobility in sodium dodecyl sulfate-polyacrylamide gels. The increase in phosphorylation was due primarily to an increase in phosphoserine, with only a minor component (

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalBlood
Volume77
Issue number2
StatePublished - 1991
Externally publishedYes

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Phosphorylation
Proto-Oncogenes
Interleukin-3
Myeloid Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Human engineering
Chemical activation
Cells
Phosphoserine
Cell Line
Signal transduction
Protein-Serine-Threonine Kinases
Sodium Dodecyl Sulfate
Signal Transduction
polyacrylamide gels

ASJC Scopus subject areas

  • Hematology

Cite this

Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce rapid phosphorylation and activation of the proto-oncogene Raf-1 in a human factor-dependent myeloid cell line. / Kanakura, Y.; Druker, Brian; Wood, K. W.; Mamon, H. J.; Okuda, K.; Roberts, T. M.; Griffin, J. D.

In: Blood, Vol. 77, No. 2, 1991, p. 243-248.

Research output: Contribution to journalArticle

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AU - Roberts, T. M.

AU - Griffin, J. D.

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