@article{dcb05b52ddb143e68493a44beeca7c72,
title = "Gradient design to optimize rate zonal separations",
abstract = "An approach to the design of gradients which maximize resolution is developed by analyzing the sedimentation of particles in linear sucrose gradients. Our analysis establishes the fundamental principles of rate separations. These principles can assist in the successful design of preparative centrifugation procedures. Rate separations are always optimal in homogeneous media or very shallow gradients of low density. In homogeneous media, resolution of particles which differ only in sedimentation coefficients is determined by the ratio of their sedimentation coefficients. Particles whose sedimentation properties oppose each other can, under certain conditions, not separate or barely separate unless conditions are carefully selected. Particle populations which differ more in density than in sedimentation coefficients clearly separate better by rate than by isopycnic banding. Rate separations in gradients are considerably improved in a type of gradient where the viscosity decreased as the density increased.",
author = "Lynn Churchill and Gary Banker and Cotman, {Carl W.}",
note = "Funding Information: The separation of particles by centrifugation depends on differences in density and sedimentation rate. Separations by density differences are achieved by isopycnic banding; separations by differences in sedimentation rates are achieved by differential or rate zonal centrifugation. Selection of media for optimal separation by isopycnic banding depends only on knowledge of the particle densities to be separated (1{\textquoteright}1. Selection of media to optimize separations by rate differences depends on knowl- {\textquoteleft}This research was supported by a grant from the National Institutes of Health (NB 68597). {\textquoteleft}Lynn Churchill (Doyle) is the recipient of a National 1nstitut.e of Mental Health Predoctoral fellowship (MH 5169741). Current address: Department, of Pharmacology, University of Wisconsin Medical Center, Madison, Wis. 53766. This article is a portion of a dissertation submitted to the University of California at Irvine in partial fulfillment of the requirements for the degree of Doctor of Philosophy. “Gary Banker is the recipient of a National Institute of Mental Health Predoctoral fellowship (MH 50166-62). Current address: Department of Anatomy, Washington University School of Medicine, St. Louis, MO. 63110.",
year = "1973",
month = dec,
doi = "10.1016/0003-2697(73)90203-0",
language = "English (US)",
volume = "56",
pages = "370--382",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",
}