GPER1/GPR30 Activation Improves Neuronal Survival Following Global Cerebral Ischemia Induced by Cardiac Arrest in Mice

Y. Kosaka, N. Quillinan, C. T. Bond, R. J. Traystman, P. D. Hurn, P. S. Herson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Female sex steroids, particularly estrogens, contribute to the sexually dimorphic response observed in cerebral ischemic outcome, with females being relatively protected compared to males. Using a mouse model of cardiac arrest and cardiopulmonary resuscitation, we previously demonstrated that estrogen neuroprotection is mediated in part by the estrogen receptor β, with no involvement of estrogen receptor α. In this study, we examined the neuroprotective effect of the novel estrogen receptor, G protein-coupled estrogen receptor 1 (GPER1/GPR30). Male mice administered with the GPR30 agonist G1 exhibited significantly reduced neuronal injury in the hippocampal CA1 region and striatum. The magnitude of neuroprotection observed in G1-treated mice was indistinguishable from estrogen-treated mice, implicating GPR30 in estrogen neuroprotection. Real-time quantitative RT-PCR indicates that G1 treatment increases expression of the neuroprotective ion channel, small-conductance calcium-activated potassium channel 2. We conclude that GPR30 agonists show promise in reducing brain injury following global cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)500-507
Number of pages8
JournalTranslational Stroke Research
Volume3
Issue number4
DOIs
StatePublished - Dec 2012

Fingerprint

Induced Heart Arrest
Brain Ischemia
Estrogens
Estrogen Receptors
Small-Conductance Calcium-Activated Potassium Channels
Hippocampal CA1 Region
Estrogen Receptor alpha
Cardiopulmonary Resuscitation
Neuroprotective Agents
G-Protein-Coupled Receptors
Heart Arrest
Ion Channels
Brain Injuries
Real-Time Polymerase Chain Reaction
Steroids
Wounds and Injuries
Neuroprotection
Therapeutics

Keywords

  • Cardiac arrest
  • Cerebral ischemia
  • G1
  • GPR30/GPER1
  • SK2

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Cardiology and Cardiovascular Medicine

Cite this

GPER1/GPR30 Activation Improves Neuronal Survival Following Global Cerebral Ischemia Induced by Cardiac Arrest in Mice. / Kosaka, Y.; Quillinan, N.; Bond, C. T.; Traystman, R. J.; Hurn, P. D.; Herson, P. S.

In: Translational Stroke Research, Vol. 3, No. 4, 12.2012, p. 500-507.

Research output: Contribution to journalArticle

Kosaka, Y. ; Quillinan, N. ; Bond, C. T. ; Traystman, R. J. ; Hurn, P. D. ; Herson, P. S. / GPER1/GPR30 Activation Improves Neuronal Survival Following Global Cerebral Ischemia Induced by Cardiac Arrest in Mice. In: Translational Stroke Research. 2012 ; Vol. 3, No. 4. pp. 500-507.
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