Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient

Gunnsteinn Hall, Steven Jacques, Kevin W. Eliceiri, Paul J. Campagnola

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Abstract

The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.

Original languageEnglish (US)
Pages (from-to)2707-2719
Number of pages13
JournalBiomedical Optics Express
Volume3
Issue number11
DOIs
Publication statusPublished - Nov 1 2012

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

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