Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient

Gunnsteinn Hall, Steven Jacques, Kevin W. Eliceiri, Paul J. Campagnola

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.

Original languageEnglish (US)
Pages (from-to)2707-2719
Number of pages13
JournalBiomedical Optics Express
Volume3
Issue number11
DOIs
StatePublished - Nov 1 2012

Fingerprint

Anisotropy
anisotropy
coefficients
scattering
simulation
attenuation
scattering coefficients
muscles
ballistics
mice
Muscles
optical properties
estimates
geometry

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient. / Hall, Gunnsteinn; Jacques, Steven; Eliceiri, Kevin W.; Campagnola, Paul J.

In: Biomedical Optics Express, Vol. 3, No. 11, 01.11.2012, p. 2707-2719.

Research output: Contribution to journalArticle

Hall, Gunnsteinn ; Jacques, Steven ; Eliceiri, Kevin W. ; Campagnola, Paul J. / Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient. In: Biomedical Optics Express. 2012 ; Vol. 3, No. 11. pp. 2707-2719.
@article{679dc84dc64f41c8b4a3600f4a1f083a,
title = "Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient",
abstract = "The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.",
author = "Gunnsteinn Hall and Steven Jacques and Eliceiri, {Kevin W.} and Campagnola, {Paul J.}",
year = "2012",
month = "11",
day = "1",
doi = "10.1364/BOE.3.002707",
language = "English (US)",
volume = "3",
pages = "2707--2719",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "11",

}

TY - JOUR

T1 - Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient

AU - Hall, Gunnsteinn

AU - Jacques, Steven

AU - Eliceiri, Kevin W.

AU - Campagnola, Paul J.

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.

AB - The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.

UR - http://www.scopus.com/inward/record.url?scp=84869134884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869134884&partnerID=8YFLogxK

U2 - 10.1364/BOE.3.002707

DO - 10.1364/BOE.3.002707

M3 - Article

C2 - 23162710

AN - SCOPUS:84869134884

VL - 3

SP - 2707

EP - 2719

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 11

ER -