Abstract
The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.
Original language | English (US) |
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Pages (from-to) | 2707-2719 |
Number of pages | 13 |
Journal | Biomedical Optics Express |
Volume | 3 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 2012 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics