Gonadotropin-releasing hormone-induced stimulation of the rat secretogranin II promoter involves activation of CREB

Seok Bean Song, Myungchull Rhee, Mark S. Roberson, Richard Maurer, Kyoon Eon Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To investigate the events involved in regulation of the secretogranin II (SgII) gene, luciferase reporter constructs were transfected into gonadotrope-derived, αT3-1 cells. DNA between -91 and -60 relative to the transcription start site was found to be required for GnRH induced SgII reporter gene activation. This region contains a consensus cAMP response element (CRE) and disruption of this CRE reduced GnRH responsiveness of the SgII promoter. CREB was shown to bind to the SgII CRE and transfection studies with a dominant-negative CREB mutant provided evidence that CREB is required for GnRH responsiveness of the SgII promoter. An expression vector for an inhibitor of the cAMP-dependent protein kinase was found to reduce the ability of cAMP or GnRH to activate the SgII-luciferase reporter gene. These studies offer evidence that GnRH-induced activation of the SgII promoter in the αT3-1 cell line requires cAMP-dependent protein kinase activity and a functional CRE within the 5′-flanking region of the gene.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume199
Issue number1-2
DOIs
StatePublished - Jan 31 2003

Fingerprint

Secretogranin II
Gonadotropin-Releasing Hormone
Rats
Chemical activation
Response Elements
Genes
Reporter Genes
Cyclic AMP-Dependent Protein Kinases
Luciferases
5' Flanking Region
Transcription Initiation Site
Transcriptional Activation
Transfection
Cells
Cell Line
DNA

Keywords

  • cAMP-dependent protein kinase
  • cAMP-response element
  • Gonadotropin releasing hormone
  • Secretogranin II

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gonadotropin-releasing hormone-induced stimulation of the rat secretogranin II promoter involves activation of CREB. / Song, Seok Bean; Rhee, Myungchull; Roberson, Mark S.; Maurer, Richard; Kim, Kyoon Eon.

In: Molecular and Cellular Endocrinology, Vol. 199, No. 1-2, 31.01.2003, p. 29-36.

Research output: Contribution to journalArticle

Song, Seok Bean ; Rhee, Myungchull ; Roberson, Mark S. ; Maurer, Richard ; Kim, Kyoon Eon. / Gonadotropin-releasing hormone-induced stimulation of the rat secretogranin II promoter involves activation of CREB. In: Molecular and Cellular Endocrinology. 2003 ; Vol. 199, No. 1-2. pp. 29-36.
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