Gonadotropin-releasing hormone-induced stimulation of the rat secretogranin II promoter involves activation of CREB

Seok Bean Song, Myungchull Rhee, Mark S. Roberson, Richard A. Maurer, Kyoon Eon Kim

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

To investigate the events involved in regulation of the secretogranin II (SgII) gene, luciferase reporter constructs were transfected into gonadotrope-derived, αT3-1 cells. DNA between -91 and -60 relative to the transcription start site was found to be required for GnRH induced SgII reporter gene activation. This region contains a consensus cAMP response element (CRE) and disruption of this CRE reduced GnRH responsiveness of the SgII promoter. CREB was shown to bind to the SgII CRE and transfection studies with a dominant-negative CREB mutant provided evidence that CREB is required for GnRH responsiveness of the SgII promoter. An expression vector for an inhibitor of the cAMP-dependent protein kinase was found to reduce the ability of cAMP or GnRH to activate the SgII-luciferase reporter gene. These studies offer evidence that GnRH-induced activation of the SgII promoter in the αT3-1 cell line requires cAMP-dependent protein kinase activity and a functional CRE within the 5′-flanking region of the gene.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume199
Issue number1-2
DOIs
StatePublished - Jan 31 2003

Keywords

  • Gonadotropin releasing hormone
  • Secretogranin II
  • cAMP-dependent protein kinase
  • cAMP-response element

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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