Gonadotropin-releasing hormone and norepinephrine release from the rabbit mediobasal and anterior hypothalamus during the mating-induced luteinizing hormone surge

Alan H. Kaynard, K. Y Francis Pau, David Hess, Harold G. Spies

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    The release of hypothalamic GnRH in association with the mating-induced LH surge was studied in the rabbit. Push-pull perfusion (PPP) of the mediobasal (MBH) or anterior (AH) regions of the hypothalamus was performed on conscious, unrestrained does for 3 h before and 5 h after exposure to a vasectomized buck. In experiment 1, GnRH concentrations were measured by RIA in 20-min fractions of MBH-PPP. An ∼100-fold increase in GnRH release was observed within 1 h of coitus (pre, 1.15 ± 0.29 pg/ml; peak, 106.67 ± 37.42 pg/ml; n = 6; P <0.05). Concomitant surges of LH and PRL in the peripheral circulation were observed. In experiment 2, GnRH and norepinephrine (NE) were measured (the latter by radioenzymatic assay) in 10-min fractions of MBH-PPP. A 218% postcoital rise in NE levels (n = 5; P <0.05) in MBH-PPP accompanied an ∼50-fold peak rise in GnRH in the same samples (pre, 1.57 ± 0.23 pg/ml; peak, 76.52 ± 50.14 pg/ml; P <0.05). MBH-NE, MBH-GnRH, LH, and PRL release began rising within 10 min of coitus. In experiment 3, GnRH was measured in 20 min fractions of AH-PPP. Coitus induced a marked rise in AH-GnRH release (precoitus, 0.31 ± 0.03 pg/ml; peak, 2.25 ± 0.80 pg/ml; n = 4; P <0.05) which differed from coitus-induced MBH-GnRH release both quantitatively (i.e. ∼7-fold increase for AH vs. ∼50-100-fold increase for MBH; 50-min lag time for AH vs.

    Original languageEnglish (US)
    Pages (from-to)1176-1185
    Number of pages10
    JournalEndocrinology
    Volume127
    Issue number3
    StatePublished - Sep 1990

    Fingerprint

    Anterior Hypothalamus
    Luteinizing Hormone
    Gonadotropin-Releasing Hormone
    Norepinephrine
    Rabbits
    Coitus
    Perfusion
    Hypothalamus

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Gonadotropin-releasing hormone and norepinephrine release from the rabbit mediobasal and anterior hypothalamus during the mating-induced luteinizing hormone surge. / Kaynard, Alan H.; Pau, K. Y Francis; Hess, David; Spies, Harold G.

    In: Endocrinology, Vol. 127, No. 3, 09.1990, p. 1176-1185.

    Research output: Contribution to journalArticle

    Kaynard, Alan H. ; Pau, K. Y Francis ; Hess, David ; Spies, Harold G. / Gonadotropin-releasing hormone and norepinephrine release from the rabbit mediobasal and anterior hypothalamus during the mating-induced luteinizing hormone surge. In: Endocrinology. 1990 ; Vol. 127, No. 3. pp. 1176-1185.
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    abstract = "The release of hypothalamic GnRH in association with the mating-induced LH surge was studied in the rabbit. Push-pull perfusion (PPP) of the mediobasal (MBH) or anterior (AH) regions of the hypothalamus was performed on conscious, unrestrained does for 3 h before and 5 h after exposure to a vasectomized buck. In experiment 1, GnRH concentrations were measured by RIA in 20-min fractions of MBH-PPP. An ∼100-fold increase in GnRH release was observed within 1 h of coitus (pre, 1.15 ± 0.29 pg/ml; peak, 106.67 ± 37.42 pg/ml; n = 6; P <0.05). Concomitant surges of LH and PRL in the peripheral circulation were observed. In experiment 2, GnRH and norepinephrine (NE) were measured (the latter by radioenzymatic assay) in 10-min fractions of MBH-PPP. A 218{\%} postcoital rise in NE levels (n = 5; P <0.05) in MBH-PPP accompanied an ∼50-fold peak rise in GnRH in the same samples (pre, 1.57 ± 0.23 pg/ml; peak, 76.52 ± 50.14 pg/ml; P <0.05). MBH-NE, MBH-GnRH, LH, and PRL release began rising within 10 min of coitus. In experiment 3, GnRH was measured in 20 min fractions of AH-PPP. Coitus induced a marked rise in AH-GnRH release (precoitus, 0.31 ± 0.03 pg/ml; peak, 2.25 ± 0.80 pg/ml; n = 4; P <0.05) which differed from coitus-induced MBH-GnRH release both quantitatively (i.e. ∼7-fold increase for AH vs. ∼50-100-fold increase for MBH; 50-min lag time for AH vs.",
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