Gonadotropin and steroid control of granulosa cell proliferation during the periovulatory interval in rhesus monkeys

C. L. Chaffin, K. M. Schwinof, Richard Stouffer

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Progesterone produced in response to the midcycle gonadotropin surge is essential for ovulation and luteinization of the primate follicle. Because cell-cycle arrest is associated with the initiation of luteinization, this study was designed to determine the dynamics and regulation of granulosa cell proliferation by gonadotropin and progesterone during the periovulatory interval in the primate follicle. Granulosa cells or ovaries were obtained from macaques undergoing controlled ovarian stimulation either before (0 h) or as long as 36 h following the administration of an ovulatory hCG bolus with or without a 3β-hydroxysteroid dehydrogenase inhibitor with or without a nonmetabolizable progestin. The percentage of cells staining positive for Ki-67, a nuclear marker for cell proliferation, decreased (P <0.05) within 12 h of hCG administration in a steroid-independent manner. Levels of cyclin D2 and E mRNA did not decline during the periovulatory interval; however, cyclin B1 mRNA was reduced significantly by 12 h. Steroid depletion increased (P <0.05) cyclin B1 mRNA at both 12 and 36 h post-hCG and was reversible by progestin replacement at 36 h. The cyclin-dependent kinase inhibitor p21Cip1 was transiently increased 12 h post-hCG, whereas p27Kip1 mRNA levels increased at 36 h in a steroid-independent fashion. These data suggest that a gonadotropin bolus inhibits mitosis in granulosa cells early (12 h) in the periovulatory interval, whereas progesterone may play a later, antiproliferative role in luteinized cells of primates.

    Original languageEnglish (US)
    Pages (from-to)755-762
    Number of pages8
    JournalBiology of Reproduction
    Volume65
    Issue number3
    StatePublished - 2001

    Fingerprint

    Granulosa Cells
    Macaca mulatta
    Gonadotropins
    Steroids
    Cell Proliferation
    Luteinization
    Primates
    Cyclin B1
    Progesterone
    Messenger RNA
    Progestins
    3-Hydroxysteroid Dehydrogenases
    Cyclin D2
    Cyclin E
    Cyclin-Dependent Kinases
    Ovulation Induction
    Macaca
    Cell Cycle Checkpoints
    Ovulation
    Mitosis

    Keywords

    • Corpus luteum
    • Granulosa cells
    • Human chorionic gonadotropin
    • Ovulation
    • Progesterone

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Gonadotropin and steroid control of granulosa cell proliferation during the periovulatory interval in rhesus monkeys. / Chaffin, C. L.; Schwinof, K. M.; Stouffer, Richard.

    In: Biology of Reproduction, Vol. 65, No. 3, 2001, p. 755-762.

    Research output: Contribution to journalArticle

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