TY - JOUR
T1 - Gonadotrophic and local control of the developing corpus luteum in rhesus monkeys
AU - Stouffer, Richard L.
AU - Chandrasekher, Yasmin Aladin
AU - Slayden, Ov D.
AU - Zelinski-Wooten, Mary B.
N1 - Funding Information:
A special thanks to Drs Robert Brenner, David Hess, Michael Melner, Srinivasa Nagalla, and Don Wolf at ORPRC, plus Dr James Hutchison at Serono Laboratories, Inc., for their interest and valuable collaborations in this research. This is ORPRC Publication No. 1857. This work was supported by NIH Grants HD20869 and HD22408 (to R.L.S.), HD18185 (TVF-EE, Hormone Assay, and Culture Core Laboratories), RR00163 (ORPRC Center) and Serono Laboratories, Inc. (to R.L.S.).
PY - 1993/11
Y1 - 1993/11
N2 - The actions of the mid-cycle gonadotrophin surge to convert the pre-ovulatory follicle into the corpus luteum are not well understood in primate species. In experiments titrating the surge duration required in macaque monkeys during artificial in-vitro fertilization (TVF)-related cycles, short (≤14 h) LH surges similar to those in rodents and domestic animals failed to initiate peri-ovulatory events. Attenuated (24 h) surges, one-half the duration ofspontaneous surges in primates, reinitiated oocyte meiosisand initial luteinization of granulosa cells. However, only surges of ≥48h sustained luteal development and functionto produce luteal phases of ∼ 13 days. Three approaches—immunocytochemistry of steroid receptors, binding of radiolabelled steroid, and reverse transcription-polymerase chainreaction of mRNA—indicate that progesterone receptor (PR), but not oestrogen receptor, is expressed in the macaque corpus luteum. Studies on cells collected before and after the gonadotrophin surge identified a novel action of LH to induce PR expression in luteinizing granulosa cells. Thus, the LH surge requirements vary between non-primate and primate species, as well as between peri-ovulatory events, and the LH surge may promote cellular recognition of paracrine and autocrine factors (e.g. progesterone) that become predominant in the developing corpus luteum.
AB - The actions of the mid-cycle gonadotrophin surge to convert the pre-ovulatory follicle into the corpus luteum are not well understood in primate species. In experiments titrating the surge duration required in macaque monkeys during artificial in-vitro fertilization (TVF)-related cycles, short (≤14 h) LH surges similar to those in rodents and domestic animals failed to initiate peri-ovulatory events. Attenuated (24 h) surges, one-half the duration ofspontaneous surges in primates, reinitiated oocyte meiosisand initial luteinization of granulosa cells. However, only surges of ≥48h sustained luteal development and functionto produce luteal phases of ∼ 13 days. Three approaches—immunocytochemistry of steroid receptors, binding of radiolabelled steroid, and reverse transcription-polymerase chainreaction of mRNA—indicate that progesterone receptor (PR), but not oestrogen receptor, is expressed in the macaque corpus luteum. Studies on cells collected before and after the gonadotrophin surge identified a novel action of LH to induce PR expression in luteinizing granulosa cells. Thus, the LH surge requirements vary between non-primate and primate species, as well as between peri-ovulatory events, and the LH surge may promote cellular recognition of paracrine and autocrine factors (e.g. progesterone) that become predominant in the developing corpus luteum.
KW - Luteal development
KW - Luteal function
KW - Luteinizing hormone
KW - Oestrogen receptors
KW - Progesterone receptors
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U2 - 10.1093/humrep/8.suppl_2.107
DO - 10.1093/humrep/8.suppl_2.107
M3 - Article
C2 - 8276942
AN - SCOPUS:0027449401
SN - 0268-1161
VL - 8
SP - 107
EP - 111
JO - Human Reproduction
JF - Human Reproduction
ER -