Glutamate uptake in mice bred for ethanol withdrawal severity

Jennifer F. Buckman, Charles K. Meshul, Deborah A. Finn, Aaron Janowsky

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Rationale: Withdrawal seizure-prone and withdrawal seizure-resistant mice were selectively bred to exhibit differences in handling-induced convulsion severity during ethanol withdrawal. The glutamatergic system has been implicated in seizure activity as well as ethanol withdrawal symptoms. Objective: This study assessed L-[3H]glutamate uptake into hippocampal synaptosomes prepared from withdrawal seizure-prone and- resistant mice. Methods: Glutamate uptake was characterized following repeated handling- induced convulsions, during acute intoxication, and during peak withdrawal following chronic ethanol exposure. Results: Hippocampal synaptosomal L- [3H]glutamate uptake did not differ between convulsion- and ethanol-naive withdrawal seizure-prone and- resistant mice. Furthermore, exposure to convulsions or to a hypnotic dose of ethanol (4 g/kg) did not alter L- [3H]glutamate uptake. However, withdrawal from 72 h of ethanol exposure significantly increased L-[3H]glutamate uptake in both mouse lines as compared to their respective ethanol-naive controls. Conclusions: These data suggest that glutamate uptake is influenced by chronic ethanol exposure similarly in both withdrawal seizure-prone and- resistant mice. The observed increases in glutamate uptake during withdrawal may be associated with compensatory mechanisms triggered by chronic intoxication and are independent of the selected differences for withdrawal severity.

Original languageEnglish (US)
Pages (from-to)174-182
Number of pages9
JournalPsychopharmacology
Volume143
Issue number2
DOIs
StatePublished - Apr 29 1999

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Keywords

  • Convulsion
  • Ethanol
  • Glutamate
  • Hippocampus

ASJC Scopus subject areas

  • Pharmacology

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